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Ｔｈｅ　ｂａｃｋｇｒｏｕｎｄ　ｉｎｔｅｒｆｅｒｉｎｇ　ａｂｓｏｒｂａｎｃｅ　ａｔ　ｔｈｅ　ａｎａｌｙｔｉｃａｌ　ｗａｖｅｌｅｎｇｔｈ　ｉｓ　
ｔｈｅｎ　ｅｓｔｉｍａｔｅｄ　ｕｓｉｎｇ　ｌｉｎｅａｒ　ｉｎｔｅｒｐｏｌａｔｉｏｎ　（ｓｅｅ　Ｆｉｇｕｒｅ　Ａ２）．Ｔｈｉｓ　ｍｅｔｈｏｄ　
ｒｅｐｒｅｓｅｎｔｓ　ａｎ　ｉｍｐｒｏｖｅｍｅｎｔ　ｏｖｅｒ　ｔｈｅ　ｓｉｎｇｌｅ－ｗａｖｅｌｅｎｇｔｈ　ｒｅｆｅｒｅｎｃｅ　
ｔｅｃｈｎｉｑｕｅ　ｂｅｃａｕｓｅ　ｉｔ　ｃｏｒｒｅｃｔｓ　ｆｏｒ　ａｎｙ　ｂａｃｋｇｒｏｕｎｄ　ａｂｓｏｒｂａｎｃｅ　ｔｈａｔ　
ｅｘｈｉｂｉｔｓ　ａ　ｌｉｎｅａｒ　ｒｅｌａｔｉｏｎｓｈｉｐ　ｔｏ　ｔｈｅ　ｗａｖｅｌｅｎｇｔｈ．　Ｉｎ　ｍａｎｙ　ｃａｓｅｓ，　ｉｆ　ｔｈｅ　
ｗａｖｅｌｅｎｇｔｈ　ｒａｎｇｅ　ｉｓ　ｎａｒｒｏｗ，　ｉｔ　ｗｉｌｌ　ｂｅ　ａ　ｒｅａｓｏｎａｂｌｅ　ｃｏｒｒｅｃｔｉｏｎ　ｆｏｒ　
ｎｏｎ－ｌｉｎｅａｒ　ｂａｃｋｇｒｏｕｎｄ　ａｂｓｏｒｂａｎｃｅｓ　ｓｕｃｈ　ａｓ　ｔｈａｔ　ｒｅｓｕｌｔｉｎｇ　ｆｒｏｍ　
ｓｃａｔｔｅｒｉｎｇ　ｏｆ　ｆｒｏｍ　ａ　ｃｏｍｐｌｅｘ　ｍａｔｒｉｘ

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Fig A2

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Summary of Contents for UV-2802

Page 1: ......

Page 2: ...tructions 4 Specifications 5 Installation 5 Operation 6 Prepare the Spectrophotometer 6 Description of keys 6 Turn on spectrophotometer 7 Basic operation 8 Analyse Sample 12 Basic Mode 12 Quantitative...

Page 3: ...and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Electrical Before s...

Page 4: ...ories Introduction The UNICO UV 2802 model spectrophotometer Fig 1 is a single beam general purpose instrument designed to meet the needs of the Conventional Laboratory The UNICOUV 2802 model spectrop...

Page 5: ...photometer light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the s...

Page 6: ...lace the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields...

Page 7: ...or scan the base line PRINT Print test results or screen START Start testing or scanning sample ESC STOP Exit to previous screen or cancel the operation ENTER Confirm the inputted data or selected ite...

Page 8: ...pass or press ESC the screen display as Fig 5 Select No to skip to main menu Fig 7 and select Yes recommended to calibrate system Fig 6 The calibrating process include get dark current searching 656 1...

Page 9: ...W Cell 1 1 2 3 8 7 4 5 6 Basic mode Quantitative WL scan Kinetics DNA Protein Multi WL Utility Defined test United Products Instruments Inc UNICO SPECTROPHOTOMETER SPECTRO QUEST Fig 7 Basic operation...

Page 10: ...ark current don t be taken after power on if you bypass the calibrating system It is recommended to take the dark current after warm up See page 38 Warning D2 W Cell 1 Max E F1 Unit F2 Mode F3 F Facto...

Page 11: ...can test For example Press in Fig 7 go into WL scan After LOAD being pressed the first file ABC wav in memory will appear on the bottom line of screen Showed as Fig 13 Press or to browse the files str...

Page 12: ...L Scan wav Kinetics kin DNA Protein dna Multi WL mul WL Validity wlv Accu Validity phv Save data or curve Example Save curve in WL scan Press the key SAVE in Fig14 to save curve Name the curve by pres...

Page 13: ...ss the key PRINT to print the report curve or data you have loaded or tested Fig 17 Fig 16 Fig 17 Before measurement Make a blank reference solution by filling a clean cuvette or test tube half full w...

Page 14: ...led or Fig 19 automatic changer uninstalled and wait for the operator ESC STOP to exit Note If no automatic changer installed cell 1 and Max E will disappear in Fig18 WL 656 1nm 12 35 27 0 000 Abs D2...

Page 15: ...mg ml F factor 2 000 Fig 21 WL 656 1nm 12 35 27 D2 W Cell 1 Max E Please input self defined unit c 0 000 mg ml F factor 2 000 Fig 22 4 Push the blank cuvette into the light path and press 0Abs 100 T...

Page 16: ...Fig 24 J Print Test Report Press PRINT to print test results Fig 25 Fig 25 Quantitative Press 2 in Main Menu for Quantitative Test Fig 26 Press ESC STOP to exit Note If no automatic changer installed...

Page 17: ...dix C for the correction method WL 700 0nm Abs 12 35 27 D2 W Cell 1 Quantitative Test ID Abs Conc mg L WL nm 700 0 C 1 000 A 1 r 1 000 Correction method Single WL Search Scroll Fig 28 3 Press F2 in Fi...

Page 18: ...on co efficients default 1 3 3 Press F3 in Fig 29 to establish a standard curve by measuring a group of standard samples See Fig 30 3 3 1 Enter standard concentrations of samples by pressing the Numer...

Page 19: ...8 and pressing ENTER to comfirm 3 3 4 Press F4 to draw the curve You can get a different curve by pressing F1 to select a different fitting method See Fig 34 Fig37 For linear fits r represent fitting...

Page 20: ...ESC STOP to exit 4 Quantitative Test Before test the standard curve must be obtained There are three ways for you to obtained it a b or c a Standard curve built up and saved in the instrument In Fig 3...

Page 21: ...WL nm 700 0 C 1 000 A 1 r 1 000 Search Scroll ID Abs Conc mg L 1 0 062 0 062 2 0 061 0 061 3 0 062 0 061 4 0 061 0 061 Fig 38 4 3 If there is more than one sample repeat step 4 2 for the next sample 4...

Page 22: ...27 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm Scan from 680 0 T 120 0 XScale YScale Fig 42 Note Scan step allows the selection of 0 1nm 0 2nm 0 5nm 1nm 2nm and 5nm Scan speed allows the...

Page 23: ...2 W Cell 1 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm XScale YScale Press ESC to stop WL 417 0nm T 36 73 Scan to 200 0nm 0 T 120 0 Fig 45 D2 W Cell 1 WL 680 0nm T 12 35 27 200 0 Waveleng...

Page 24: ...ss F3 to search the Abs T value of the scan There are two ways for you to search Fig 49 D2 W Cell 1 WL 680 0nm T 12 35 27 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm Point Peak F1 Set Pea...

Page 25: ...int to point Press to search the point from left to right and press to search from right to left The search step interval is the same as the scan step The value of every point searched will be display...

Page 26: ...25 Fig 52 Kinetics Press 4 in main menu for Kinetics Fig 53 ESC STOP to exit To load a previous kinetics result press LOAD and select a previously stored result kin...

Page 27: ...bs 3 000 XScale YScale Total Time 180 Fig 54 Select the test mode Abs or T by pressing F2 Fig 55 D2 W Cell 1 Tim 60s Abs 12 35 27 0 Time s 180 Total T 180s Inteval 1 0s 0 Abs 3 000 XScale YScale Pleas...

Page 28: ...The gradient of this line gives the rate of change of A min Note I U Factor A min D2 W Cell 1 Tim 60s Abs 12 35 27 0 Time s 180 Total T 180s Inteval 1 0s 0 Abs 3 000 XScale YScale Begin Time 0 Fig 57...

Page 29: ...y page Fig 56 Press ESC if in Search to return to the required page J Print Test Report Press the key PRINT to print the curve you have loaded or scanned Fig 59 Fig 59 DNA Protein Press 5 in main menu...

Page 30: ...61 D2 W Cell 1 Input f1 62 90 DNA Protein measurement No Items Result Unit WL 900 0nm 12 35 27 WL nm 260 0 280 0 320 0 Search Scroll Fig 61 2 Press F2 to select test mode Absorbance difference 1 is f...

Page 31: ...Pull the sample cuvette into the light path press START to test the sample The test result will be displayed on the screen Fig 65 D2 W Cell 1 F1 Coeff F2 Mode F3 Unit F4 Default DNA Protein measureme...

Page 32: ...the key F4 to recall the default of the f1 f4 J Save Data Press the key SAVE to save data J Print Test Report Press the key PRINT to print the test result Fig 67 Fig 67 Multi Wavelength Press 6 in ma...

Page 33: ...h Test WL 900 0nm Abs 12 35 27 2 WL Search Scroll Please input wl 500 No WL nm Abs 500 0 400 0 546 0 Fig 69 2 Press F2 to select mode Fig 70 D2 W Cell 1 Multi Wavelength Test WL 900 0nm Abs 12 35 27 3...

Page 34: ...t sample Note When the test has finished the wavelength will go to the first WL 6 Press or for searching Input the sample number the result will be displayed on the screen Press or to browse the test...

Page 35: ...ell 1 1 2 3 9 8 4 6 7 WL Reset Printer Lamp Clock Accu Vaidity WL Vaidity Connect to PC Beeper on off United Products Instruments INC 5 Dark current F1 Delete entire saved files F2 Restore default WL...

Page 36: ...2 W Cell 1 1 2 3 4 Reset printer Select print port Select printer Print report Printer HP PCL 1 color cartridge Fig 77 4 Press 4 in Fig 75 to select print mode If you select Print screen mode a little...

Page 37: ...et D2 lamp usage time Switch W United Products Instruments INC Reset W lamp usage time 5 Switch point Switch D2 OFF OFF Fig 80 2 Press 2 in Fig 79 to reset usage time of D2 Fig 81 Press or to select Y...

Page 38: ...OMETER SPECTRO QUEST D2 W Cell 1 1 2 3 4 Switch D2 Reset D2 lamp usage time Switch W 5 hrs used Are you sure Reset W lamp usage time 5 Switch point ON ON NO Fig 83 5 Press 5 in Fig 79 to set the switc...

Page 39: ...e mode Please input the time 08 04 35 Show Date mode D2 W Cell 1 Fig 86 2 Press 2 in Fig 85 to modify date by pressing the numeric keypad 3 Press 3 in Fig 85 to set the date display on the top right c...

Page 40: ...W Cell 1 Photometric Validity Test No WL nm Abs Std Abs Result WL 900 0nm Abs 12 35 27 F1 Standard F2 Mode F3 Tolerance Fig 89 1 Press SET to set the wavelength Press ENTER to edit and input wavelengt...

Page 41: ...lue by pressing the numeric keypad D2 W Cell 1 Photometric Validity Test WL 900 0nm T 12 35 27 No WL nm T Std T Result 1 500 0 9 28 2 600 0 8 45 3 700 0 5 66 Input tolerance 0 005 Fig 93 5 Press 0Abs...

Page 42: ...gth Validity Test WL 900 0nm Abs 12 35 27 No WL nm Peak nm T Result F1 Set peaks F2 Mode F3 Tolerance Fig 95 1 Press F1 to set the standard peak Press ENTER to edit and input wavelength by pressing th...

Page 43: ...path Press START to check The results will be displayed on the screen Fig 99 If the discrepancy between the results and the calibrated values is not more than the tolerance pass will be displayed afte...

Page 44: ...C SPECTRO QUEST Fig 100A J Beeper on off Press 9 in Fig 73 to turn on off the beeper J Delete entire saved files Press F1 in Fig 73 to delete entire saved files After the delete the files double confi...

Page 45: ...T Fig 103 D2 W Please select mode Abs 4 Ref 4 Sample No Abs 1 2 3 4 WL 900 0nm 12 35 27 0 000 Abs Fig 103 3 Put the reference into cell NO 1 and 4 samples into cell NO 2 NO 5 Set wavelength 4 Press ST...

Page 46: ...bs F1 Methord F2 Mode WL 900 0nm Place 1st group Fig 105 D2 W Cell 1 1 Ref 1 Sample No Abs 1 2 3 4 5 6 7 8 0 000 Abs F1 Methord F2 Mode WL 900 0nm Place 2nd group Fig 106 8 Remove 8 references and put...

Page 47: ...Protein concentration A2 Aref f3 A1 Aref f4 f1 62 9 f2 36 0 f3 1552 f4 757 3 Absorbance difference 260 230 A1 A260nm A2 A230nm Aref A320nm optional DNA concentration A1 Aref f1 A2 Aref f2 Protein con...

Page 48: ...ument VERY IMPORTANT HIGH VOLTAGE 2 Remove the cuvette holder rod by unscrewing the rod counterclockwise 3 Remove the all screws around the sides of the spectrophotometer See Fig A1 Fig A1 4 Very care...

Page 49: ...he instrument carefully remove 3 connectors CZ6 CZ4 and J3 on PCB marked SST8 417 100 Be sure to reconnect after replacing the lamp Fig A3 CZ6 Fig A3 5 Remove the grey metal protection cover Using scr...

Page 50: ...er to the side See Fig A4 Fig A4 6 Disconnecting the connector J7 on the PCB marked SST8 411 128 Unscrew the screw that holds the lamp bracket to the instrument base Pull the entire lamp and lamp hold...

Page 51: ...a lamp Fig A6 provided by UNICOor an authorized UNICOService Provider This comes pre assembled with lamp socket Fig A6 CAUTION THE LAMP MAY BE HOT TAKE PRECAUTIONS TO PREVENT POSSIBLE BURNS 8 Reconnec...

Page 52: ...V 2802 without the covers with high voltages accessible and so should only be performed by a suitably qualified engineer If adjustment is required remove the cover and grey protection cover put on UV...

Page 53: ...le of the rear panel You can now look through the hole and view the image of the lamp on the slit Check the lamp alignment Fig A9 If the image is not covering the slit the lamp alignment needs adjustm...

Page 54: ...from your fingers can cause the lamp to burn out prematurely 5 Re fit all the screws around the sides of the spectrophotometer Fig A1 6 Install the gray metal protection cover and cover of instrument...

Page 55: ...54 Appendix C...

Page 56: ...55 Fig A2...

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