Thermo Scientific Accela PDA, Hardware Manual

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5
ChromQuest Diagnostics for the PDA Detector
Monitoring Lamp Performance from the ChromQuest Data System
Thermo Scientific Accela PDA Detector Hardware Manual 65
Monitoring Lamp Performance from the ChromQuest Data System
With use, the deuterium lamp emits less and less light. As the light output from the
deuterium lamp decreases, the detector noise increases. For information on the typical lamp
lifetime, see
“Controlling the Lamps from the ChromQuest Data System” on page 63 .
To monitor and track lamp performance from the ChromQuest data system, follow these
procedures:
• Viewing an Intensity Scan from the ChromQuest Data System
• Recording the Performance of the Lamps
Viewing an Intensity Scan from the ChromQuest Data System
Y To monitor lamp performance from the ChromQuest data system
1. Open the Instrument window for your LC system:
a. From the computer desktop, choose Start > All Programs > Chromatography >
ChromQuest
.
The Main Menu window appears.
b. Double-click the icon that represents your LC stack.
The Instrument window appears.
2. Create and download a method that pumps 100% HPLC-grade methanol or
HPLC-grade water at a constant flow rate of 1 mL/min.
3. Turn on the lamps and check their status (see “Controlling the Lamps from the
ChromQuest Data System” on page 63 ).
Wait for one hour for both lamps to equilibrate.
4. Open the Accela PDA Diagnostics dialog box (see “Accessing the Direct Controls for the
Accela PDA Detector” on page 62 ).
5. Click the Control tab.
The Control page appears.
6. In the Mode area, select the Intensity option.
7. Click Default , and then verify that the following parameters are specified in the
Spectrum area (see Figure 56 ):
Start = 2
End = 511
Step = 1