Thermo Scientific Accela PDA, Hardware Manual

Page: 119 / 128

7
Troubleshooting
Detector-Related Problems
Thermo Scientific Accela PDA Detector Hardware Manual 99
No peaks, or peaks
much smaller than
expected. Incorrect wavelength setting. Check the wavelength setting. Make sure the
correct file is selected.
Lamp not on or defective. Make sure the lamp is lit. Verify the lamp
performance. See one of the following:
• “Monitoring Lamp Performance from the
Xcalibur Data System”
on page 38
• “Monitoring Lamp Performance from the
ChromQuest Data System”
on page 65
Replace the lamp if necessary.
Integrator input voltage does not match
detector output voltage. Verify that the integrator is connected to
appropriate Analog Output connections on the
detector ‘s back panel. (See
Chapter 2,
“Installation.” ) Check the attenuation setting on
the integrator.
Insufficient sample reaching the
detector. Check the entire chromatographic system for
leaks. Check the sample injection volume.
Broad, tailing peaks. Rise time is too long. Lower the rise time selection.
Poor connection at LightPipe flowcell
inlet. Check the end of the inlet tubing for a clean, flat
surface that is free of obstructions.
Detector does not
power up. Tripped circuit breaker at power outlet. Resolve the problem; reset the circuit breaker.
Blown detector fuse. Resolve the problem; replace the fuse (see
“Replacing the Fuses” on page 95 ).
Power cord not connected. Connect the power cord.
Detector does not go
into run upon
injection. Detector not receiving Start signal. Check the connection to the Run contacts on the
back panel of the detector.
Flat baseline, portion
of spectrum missing. Saturation of photodiode array. Adjust the attenuators. See one of the following:
• “Adjusting the Light Throughput with the
ChromQuest Data System”
on page 69
• “Adjusting the Light Throughput with the
Xcalibur Data System”
on page 42
Power LED is amber Ethernet connection may be loose. Check the Ethernet connection to the data
system computer.
Comm LED is amber Connection to the data system
computer has been lost. Check the Ethernet connection to the data
system computer.
Table 5. Detector-related problems (Sheet 3 of 4)
Symptom Cause Remedy