Thermo Scientific Accela PDA, Hardware Manual

Page: 96 / 128

5
ChromQuest Diagnostics for the PDA Detector
Calibrating the PDA Detector from the ChromQuest Data System
76 Accela PDA Detector Hardware Manual Thermo Scientific
3. Open a wavelength calibration file:
a. In the Wavelength File area, click Open .
The Select Wavelength Calibration dialog box appears (see Figure 63 ).
Figure 63. Select Wavelength Calibration dialog box
b. Select an appropriate wavelength calibration file from the list.
An appropriate wavelength file should include the range of wavelengths that you use
under normal operating conditions.
c. Click Open .
The holmium oxide bands of the selected file appear in the Wavelength File area
(see
Figure 64 ).
Note ChromQuest has four calibration files to choose from. The HolmiumUV
file contains five wavelengths in the UV region while the other files, such as
Holmium12, use sets of wavelengths from both the UV and Visible wavelength
regions. The holmium oxide absorbance maxima are selected from a spectrum
published in “Holmium Oxide Solution Wavelength Standard from 240 to
640 nm - SRM 2034 (NIST Special Publication 260-54).”