© Thermo Hybaid. Issue 1.0, January 2002
34
•
Temperature range achieved.
•
The actual sample temperature as defined by the tube thermistor in position A2 of the block (when
Active Tube Control has been selected for a run).
Power Failure Screen
This additional screen appears if the run has been interrupted by a power failure or power fluctuation
severe enough to affect the unit. It contains the following information: -
•
The duration of the power failure.
•
The stage in the run when the power failure occurred.
End of Run Screen
The Px2 displays a further screen at the end of the run, which provides the following:
•
The program name and number.
•
The total run time.
•
The maximum and minimum temperatures recorded during the run.
•
Power interrupt information if a power failure occurred.
Error Screens
A number of error screens may be displayed if a fault has been detected in the unit. Contact the
Thermo Hybaid Service Department or your local supplier for advice before attempting to use the
instrument further.
6.4 Temperature Control Options
Crucial to the accurate operation of a thermal cycler is an understanding of the temperature control
methodology.
Active Tube Control (ACTIVE TUBE)
Recommended for all reactions above 20
µ
l volume in 0.5ml and 0.2ml tubes. This type of control
uses the remote thermistor probe mounted in an appropriate tube. A volume of mineral oil equivalent
to the total volume in the reaction tubes (including any oil overlay) must be present in the control tube.
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