© Thermo Hybaid. Issue 1.0, January 2002
59
APPENDIX II
Px2 THERMAL CYCLER
Sealing Systems: The OmniSeal TD Mat & OptiTape
Overview
Thermo Hybaid offers several sealing systems to suit individual preferences. OptiTape is an optically
clear single use adhesive tape suitable for use with 96 well polycarbonate plates, whilst the OmniSeal
TD Mat is a reusable silicone rubber mat also for use with 96 well plates. For polypropylene plates
we supply adhesive TDX Tape.
How to use the OptiTape and TDX Tape
OptiTape
and
TDX Tape
are unique, single use sealing systems, designed for oil-free thermal
cycling in 96 well plates and to prevent cross contamination between wells. After a thermal cycling
reaction OptiTape or TDX Tape can be removed from the plate with ease.
OptiTape
Supplied on a roll (with optional dispenser) and designed for use with Thermo Hybaid’s OmniUltra
Plate, although will give an exceptional seal with other brands of
polycarbonate
plate.
TDX Tape
Clear in colour and designed for use with
polypropylene
plates such as OmniTube 96, OmniFast 96
or OmniFast 384.
OptiTape and TDX Tape cannot be interchanged, as the TDX Tape will bind irreversibly to
polycarbonate.
To secure contact with the heated lid of the thermal cycler the OptiTape and TDX Tapes must be
used in combination with the reusable TD Compression Pad.
Sealing a 96 Well Plate
1. The tape can be applied to 96 well plates by positioning over one of the short edges and peeling
back the lining material, allowing the adhesive layer to fall on to the plate. This should be possible
without introducing creases. However, before heating, the adhesive is soft enough to be
repositioned without adverse effects.
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