© Thermo Hybaid. Issue 1.0, January 2002
26
Enter and change Advanced Edit parameters using the number, arrow and
ENTER
keys. The
Advanced Edit feature can also be accessed from the normal EDIT program menu options if
Advanced Edit parameters are to be added to an existing program.
If desired, the Advanced Edit feature can be deleted for the current step. To do this, set all the
parameters back to zero. The
<ADV>
message will then not be shown in the temperature/time set up
screen, denoting that no Advanced Edit functions are operating.
Time Advanced Edit Worked Example
The user can specify the increment/decrement per cycle for each program stage.
Time increments can be used for example in
high cycle number reactions
to allow longer for
enzyme action with successive cycles.
Below is an example of a protocol where the extension step is fixed for the first 15 cycles of a 25-
cycle program and increased by 5 seconds/cycle for the next 10 cycles, to compensate for loss of
enzyme activity:
Stage 1:
Enter parameters to create:
95
o
C - 30s
55
o
C - 30s
x 14 Cycles
72
o
C - 30s
Stage 2:
Enter parameters to create:
95
o
C - 30s
55
o
C - 30s
x 11 Cycles
72
o
C - 30s + 5s/cycle
1. To enter the +5s/cycle, select
PROG
followed by
EDIT
.
2. In the
EDIT
mode (NEW or EXISTING) move Stage 2, Step 3 (relevant extension step) using
the navigation keys.
3. Press
ADVANCED
to access the Advanced Edit screen.
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