© Thermo Hybaid. Issue 1.0, January 2002
22
Note: If there are no free program spaces the Px2 will prompt you to overwrite an
existing program.
7. Enter temperature (e.g. 95
°
C) and time (hr:min:sec), as required. Use the
LEFT
softkey to move
the cursor as desired or
ENTER
to advance to the next step. The
UP
softkey can be used to
scroll backward to correct a mistake. The program will not allow you to advance to step 2 until a
valid temperature and time have been entered.
Note:
Pressing the
ADVANCED
softkey at any step throughout the programming
provides access to the advanced edit features. These facilitate the creation of more
complex protocols including touchdown or gradient methods (see section 5.4 for more
details).
8. Continue to enter the desired time and temperature STEPS of this STAGE. If no more STEPS
are required in the STAGE press
ENTER
(leaving each parameter at 0:0) until you access the
number of cycles screen.
9. Enter the number of times you would like to cycle through the STAGE using the alphanumeric
keys. A hold step can also be programmed after each stage so once the cycles have been
completed the Px2 will wait at the specified temperature until manually advanced. This is
particularly useful for ‘hot start’ protocols. If a hold step is not required, press
ENTER
to reach
the next stage.
10. Continue adding steps to the new stage as per stage 1.
11. When all the steps have been entered in the last stage entering values of 0:0 will again bring access
to the number of cycles required screen. Enter the number of cycles. Enter a hold temperature if
required. If a hold temperature is not entered the instrument will cool to room temperature once
the protocol is finished. When
ENTER
is pressed, a new STAGE and STEP is displayed. By
ENTERING values of 0:0 in the first step of a new stage the SAVE screen is accessed.
12. Press
SAVE
to save the program or
DISCARD
to abort the program. Pressing MENU at any
time aborts the programming.
Editing an Existing Program
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