6. LSServer, Scanner Shell and Download
2004-11
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
6-77
Gain Calibration…
To start the
Gain Calibration wizard
, select
Gain Calibration…
in the Service
menu.
A wizard guides the user through the gain calibration procedure.
The
Gain Calibration
option is implemented in
Scanner Shell
as a wizard. It
represents workflow modules, which define a sequential procedure and guide the
user step-by-step to perform a specific complex activity.
The
Gain Calibration
is needed
to
take into account differences in sensitivity
between PMTs used in
Tecan LS SERIES SCANNER
instrument. It is NOT only
necessary for dual channel instruments. The deviation between PMTs can be up
to 500 %.
The
LS SERIES SCANNER
is provided with calibrated PMTs. Data from the
PMTs becomes less accurate over the course of its life-span, so gain calibration
should be repeated regularly: After
100 days
the user will be requested to start
gain calibration again.
Note
Gain calibration no longer valid after 100 days.
Tools needed for gain calibration are delivered with each instrument:
•
Scattered light glass slide
•
Calibration filter slide with different OD filters
For further information refer to the chapter The Gain Calibration Wizard on page
6-77 in this manual.
During the
Gain Calibration
procedure:
1. Door opens and filter slides are moved out.
2. The user is asked to prepare the instrument for
Gain Calibration
.
3. Instrument parameters are set for
Gain Calibration
.
4.
Gain Calibration
PMT1 is done.
5.
Gain Calibration
PMT2 is done.
6.
Gain Calibration
data PMT1 / PMT2 is saved
as GainCalibration.cvs in the CalData folder.
7. Door is closed and filter slides are moved out.
Note
The gain calibration must be done for all PMTs found in instrument.
