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tecan ls series, Operating Manual

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6.  LSServer, Scanner Shell and Download 

 

6-2 

Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5  

2004-11 

6.1.3 Software 

Requirements 

The following software requirements are needed for 

LSServer

 and

 Scanner 

Shell

: 

Operating system 

Minimum 

Recommend 

Microsoft Windows 

Windows 2000 

Windows 2000  

Additional supported software: 

Array Pro 4.5

 for microarray image analysis. 

 

Note 

Microsoft®Excel and Microsoft®Word must be registered by the user!  

6.1.4 

Software Installation Procedure 

Automatic Software Setup 

LSServer

 / 

Scanner Shell

 and 

LSDownload

 are installed by an automatic 

software setup program: 
 

 

Note 

Before installing LSServer components be sure that Array Pro 4.5 

Software for Microarray Image Analysis is installed on your system.  

 

 

Note 

It is necessary that the person who installs the software has administrator 

rights on a PC with a Win 2000 operating system.  

 

Step Procedure 

1. 

Place the LS Driver for Tecan LS V2.2 Setup CD in the CD-ROM 
drive. 

2. 

Open the CD drive folder and start

 

LSServer.exe

. The installation 

procedure begins. 

3. A 

Welcome

 window informs the user about the beginning of the 

installation process. Click 

Next

 to continue. 

4. 

A Read Me File window informs the user about the new features of 
the setup. 

5. 

The installation of the required files is carried out and files are copied 
to the computer. At the end of a successful installation the user can 
quit the installation wizard by clicking the 

Finish

 button. 

 
After successful installation, installed software components for LSServer can be 
started via the 

Window Start menu 

by selecting 

Start - Programs - Array-Pro 

Analyzer 4.5 - Array-Pro Analyzer

 and installed software components for 

Scanner Shell and LSDownload can be started via the 

Window Start menu 

by 

selecting 

Start - Programs - Tecan - ScannerShell

 or 

LSDownload

. 

Summary of Contents for ls series

Page 1: ...TECAN Operating Manual for the LS Series Scanner Document Part No 30001798 2004 11 Document Revision No 1 5...

Page 2: ...ax 49 79 51 50 38 Italy Tecan Italia S r l Via F lli Cervi Palazzo Bernini Centro Direzionale Milano2 20090 Segrate Mi Italy Tel 39 02 215 21 28 Fax 39 02 215 97 441 Japan Tecan Japan Co Ltd Meiji Sei...

Page 3: ...A 5082 Gr dig Salzburg AUSTRIA EUROPE Telephone 0043 0 6246 8933 FAX 0043 0 6246 72770 E mail office austria tecan com Copyright Information The contents of this document are the property of Tecan Aus...

Page 4: ...e notices appear below Note Gives helpful information Caution Indicates a possibility of instrument damage or data loss if instructions are not followed WARNING INDICATES THE POSSIBILITY OF SEVERE PER...

Page 5: ...s 4 3 4 3 Data Processing 4 3 5 Operation 5 1 General Operating Features 5 2 5 1 1 Instrument Start Up 5 2 5 1 2 Instrument Power On 5 2 5 1 3 Connect to Instrument 5 3 5 1 4 Finish a Measurement Sess...

Page 6: ...Unlock Wizard 6 95 6 5 LSDownload 6 101 6 5 1 Starting LSDownload 6 101 6 5 2 LSDownload Menus 6 102 7 Instrument Features 7 1 Specifications 7 1 7 1 1 Lasers 7 4 7 1 2 Optional Barcode Scanner 7 4 7...

Page 7: ...manual may result in damage to the product injury to operating personnel or poor instrument performance 3 Observe all WARNING and CAUTION statements in the manual 4 Never open the LS SERIES SCANNER wh...

Page 8: ......

Page 9: ...t modes They can read samples up to the size of microplates as well as thick samples like gels or for instance liquid cells They can read both in confocal and in non confocal mode and a selection of u...

Page 10: ...le formats can be measured as well The software allows special formats to be defined and special scanning areas to be processed Due to an exceptionally high working distance of 6 5 mm thick samples we...

Page 11: ...for each excitation wavelength or laser respectively Additional filters can be added into the filter slides The filter slides can be exchanged by the user The identity of each filter slide is automat...

Page 12: ...SCANNERS come with an integrated array analysis software package Caution If the instructions given in this manual are not correctly carried out the instrument May either become damaged or May no Longe...

Page 13: ...RS232 cable and 1 interface cable Slide Adapters Filter Slide Reference slides histological section of mouse spots Array Pro Software Hardware Key Array Pro SW Installation CD Array Pro SW Array Pro U...

Page 14: ...a location to place the instrument that is flat level vibration free away from direct sunlight and free from dust solvents and acid vapors Allow at least 10 cm between the instrument and the wall or...

Page 15: ...sories against the packing list Save packing materials and transport locks see next section for further transportation purposes Caution Do not try to open or close the instrument flap manually Ensure...

Page 16: ...supplied to the computer is correct according to the specification input range selector red button at the power supply Caution Check voltage specification on the computer BEFORE switching ON If comput...

Page 17: ...canner No 30001798 Rev No 1 5 3 5 The pictures below show the connection of the interface cables to the back of the computer RS232 to instrument Connection for monitor cables Digital monitor cables An...

Page 18: ...r socket in the back panel of the instrument All connected devices must be approved and listed with regulations EN 60950 UL 1950 or CSA C22 2 No 950 for Data Processing Devices Switch the computer ON...

Page 19: ...chapter 6 4 10 The Transport Unlock Wizard Caution Do not try to open or close the instrument flap manually Caution Save packing materials and transport locks for further transportation purposes The...

Page 20: ...Insert the Filter Slides into Filter Channel 1 vertical orientation and Filter Channel 2 horizontal orientation and make sure that the filters are oriented properly see pictures below Filter Channel 1...

Page 21: ...butor Ensure that a fuse of the correct type and rating is fitted Fuse T 2 0 A 250 V slow blow UL recognized WARNING RISK OF FIRE REPLACE ONLY WITH FUSES OF THE SAME TYPE AND RATING Caution When insta...

Page 22: ......

Page 23: ...ure 4 1 For excitation of fluorescence signals up to 4 lasers are installed The lasers for 633 nm and 594 nm are Helium Neon lasers the lasers for 488 nm and 532nm are solid state lasers Both of these...

Page 24: ...scheme Three pinholes of different sizes are mounted on an automatic slide carrier The smaller the pinhole the lower the focal depth of the detected volume The larger pinholes typically create images...

Page 25: ...The sample is then brought into the exact focal plane both with regard to z as well as the two angles of tilt This process is fully automated Signals are recorded in reflection mode and with a dimmed...

Page 26: ......

Page 27: ...ion For details of the data analysis see the corresponding Array Pro software manual Suggestions are made on how to optimize the instrument parameters for your applications Every effort has been made...

Page 28: ...ing the instrument ON the following actions are induced The filter slides move into the reference position The dichroic filter carrier moves into the reference position The pinhole carrier moves into...

Page 29: ...eration 5 1 4 Finish a Measurement Session Disconnect from Instrument When disconnecting communication between the instrument and the PC is terminated Note Please remove the recently measured sample b...

Page 30: ...tation in the adapter If it is not placed correctly in the adapter the autofocus algorithm might fail to find a focal plane Place slide adapter on the plate transport in the same orientation as shown...

Page 31: ...Functions Connecting to Tecan LS Series Scanner Preparing measurement parameters in order to get the best results in scanning Calibrating LS Series Scanner instruments Creating special formats for sca...

Page 32: ...he software has administrator rights on a PC with a Win 2000 operating system Step Procedure 1 Place the LS Driver for Tecan LS V2 2 Setup CD in the CD ROM drive 2 Open the CD drive folder and start L...

Page 33: ...cannerShell LSDownload icon and click Remove 4 The Uninstall wizard is started Select the preferred uninstall method Automatic or Custom and click Next to continue 5 In the next window press Finish to...

Page 34: ...Setup dialog box serves to set up options for connecting instruments The desired instrument and the communication port are chosen The demo mode provides the possibility to simulate any Tecan instrumen...

Page 35: ...y available port Demo mode option button Allows the simulation of any instrument e g for test purposes Refer to chapter 6 4 6 Service Menu Demo Mode Allowed for enabling the demo mode Stacker Port gro...

Page 36: ...e first time the Tecan LS Scanner Interface dialog box is opened After selecting instrument type and communication port and confirming with OK the Measurement Control dialog box is displayed For all f...

Page 37: ...utton Click the Start button to start a measurement with the current measurement parameter The Status dialog box is displayed Prescan button Click the Prescan button to select three different prescan...

Page 38: ...apter Format group contains the following elements Load button Click the Load button to open the Open Slide Adapter Format dialog box SAF can be selected Slides to Process The Slides to process group...

Page 39: ...tion dat files Gain calibration data is stored as a comma separated list cvs files For analysis these files can be loaded in Excel or another calculation program Crosstalk calibration dat Crosstalk da...

Page 40: ...xtension Directory Measurement parameter lsp Parameter Slide adapter format saf SAF Gain calibration dat csv CalData Crosstalk dat CalData Temporary Files Temporary files needed for diagnostic and con...

Page 41: ...the Time group box the Elapsed time of the measurement and the Estimated run time are shown In the Gain control group box the gain of the PMT can be adjusted online during the measurement The display...

Page 42: ...nk button Click Shrink to minimize the display of the Status dialog box Default button Click Default to maximize the display of the Status dialog box Break button Click Break to abort the current meas...

Page 43: ...Time group box Elapsed time static text box Estimated run time static text box Gain control group box Gain 1 slider spin control Use the slider or spin control to online adjust the gain in Channel 1 G...

Page 44: ...s also possible to set the Barcode Area Click Apply to confirm the newly defined areas Click OK to save the settings and close the dialog box The Image Prescan dialog box contains the following elemen...

Page 45: ...a boundary place the cursor along the edge or corner of the AOI and drag it when the 2 way cursor appears To move the AOI place the cursor in the middle of the rectangle and drag it when the 4 way cu...

Page 46: ...area is from 37 5 mm to the total slide length If your instrument is equipped with a barcode bottom reader the barcode area is fixed The barcode label has to be placed at the upper end of the slide Th...

Page 47: ...therefore lead to incorrectly calculated gain values To start a scan with the AGC feature first of all a special area for AGC must be selected This can be done from either the Geometry Parameters tab...

Page 48: ...discarded Range 0 01 1 Signal level mean value of all pixels in the selected signal level range will be set to 50 000 Range 0 01 1 Amount of pixels Signal intensity 50 000 Noise level Signal level 65...

Page 49: ...The result is an automatic increase of the gain value Autofocus Settings group box Autofocus Amplitude mm Enter the amplitude in mm into the Autofocus Amplitude text box Select the appropriate autofoc...

Page 50: ...yed traverse path it is possible to define the slide areas Enter the correct adapter height and sample height The chosen sample height of this dialog is taken as default height for the following adapt...

Page 51: ...image is displayed The currently active saf file is displayed as an overlay on the adapter prescan image The red rectangles represents the available scan areas The big green rectangle represents the o...

Page 52: ...Click Next to save data temporarily and perform the second part of the wizard Cancel button Click Cancel to close the dialog box The selected options are not saved Help button Click Help to display t...

Page 53: ...mporally and perform the first part of the wizard Finish button Click Finish to save data and close the wizard Cancel button Click Cancel to close the dialog box The selected options are not saved Hel...

Page 54: ...focus mode selected Please select focus mode in Advanced Autofocus dialog Fluorescence focus scans will be performed with the laser filter setting of channel 1 This focus scan mode can be relevant fo...

Page 55: ...ether with an overlay of lines representing the Baseline blue Identified scanning plane red and Focus Offset green Manual Focus radio button Changes in the three combo boxes changes the plane that wil...

Page 56: ...owing elements Plate In button Click the Plate In button to close the door and to move in the plate transport Plate Out button Click the Plate Out button to open the door and to move out the plate tra...

Page 57: ...box to activate the 633 nm laser Red LED is switched on at the rear of the instrument Laser 2 532 nm check box Select Laser 2 check box to activate the 532 nm laser Green LED is switched on at the re...

Page 58: ...utofocus Settings The Measurement Parameter dialog box appears The Measurement Parameter dialog box contains the following elements General Tab Geometry Parameter Tab Scan Settings Tab Depending on th...

Page 59: ...he type of Scan mode can be selected as well as the Barcode reader and the Stacker options for automation purposes The user can also select the option of having the plate move out of the instrument af...

Page 60: ...ed and are measured simultaneously This requires dual channel instruments as detection is performed in both channels at once and scanning is done in half the time The Dual Parallel Scan Mode needs a C...

Page 61: ...cified reading position The barcode appears automatically as part of the filename see Slide Settings Geometry Parameter Tab In the Geometry Parameters tab the Scan area the location of the Autofocus s...

Page 62: ...it in mm For more information see Autofocus on page 6 35 Select Autofocus to make the Advanced Autofocus button available and to change autofocus parameters or to select a different autofocus and or a...

Page 63: ...in saf files see Advanced Menu Edit SAF on page 6 85 1 If the Adapter height is measured from the surface of the plate transport to the surface of the spots the Slide Thickness must be set to 0 2 If...

Page 64: ...following elements Top spin control Use the Top spin control or type in a value to set the upper border of the scan area The value is measured in mm from the lower left corner 0 0 Left spin control Us...

Page 65: ...tom line 0 0 Bottom spin control Use the Bottom spin control or type in a value to set the autofocus scan line for Focus 1 The value is measured in mm from the bottom line 0 0 Amplitude spin control U...

Page 66: ...barcode reading position blue line The barcode position is centered on the slide and must be at least 37 5 mm from the bottom line The barcode reading position can not be adjusted when using a barcod...

Page 67: ...area The value is measured in mm from the lower left corner 0 0 Right spin control Use the Right spin control or type in a value to set the right border of the scan area The value is measured in mm f...

Page 68: ...gain for the PMT is set respectively in one or two channels Pinhole Crosstalk correction Integration time Sensitivity and Scan Resolution can be defined Click OK to save the changes and close the dial...

Page 69: ...from the Filter Channel 1 combo box Filter Channel 2 combo box Select the appropriate filter from the Filter Channel 2 combo box to match with the selected excitation wavelength in Laser Channel 2 Al...

Page 70: ...s confocal focal depth 300 m Large non confocal focal depth 800 m The small pinhole gives high background rejection and better signal background ratio If samples are bulky or have a large volume large...

Page 71: ...he Crosstalk list box Clicking on a Crosstalk list item sets the K factor values appearing in K1 K2 static text box which will be used when starting the next measurement if the Crosstalk correction ch...

Page 72: ...Higher signal to noise ratio higher sensitivity Not comparable signal values between scans with different amplitudes Comparable range of amplitudes 22 mm 20 mm 15 mm 10 mm 5 mm Sensitivity Sensitivity...

Page 73: ...ck OK to close the dialog box and save the selected options Plates group box Read plate barcode Enabled if Tecan Connect is installed with barcode reader If the checkbox is checked a plate barcode wil...

Page 74: ...vanced Autofocus available Click the Advanced Autofocus button and the Advanced Autofocus dialog box appears Window Elements The Advanced Autofocus dialog box The Advanced Autofocus dialog box contain...

Page 75: ...ea are 800 m The range considers the tolerance of the slide thickness the tolerances of positioning the slide adapter to the instrument as well as internal tolerances of the instrument itself However...

Page 76: ...s default focal plane but any of three can be selected Plane 1 option button Select Plane 1 option button to select Plane 1 signal intensity as focal plane calculated in the order from top to bottom f...

Page 77: ...utofocus option button Select HS Autofocus option button to perform the so called five point autofocus To determine the right focal plane the plate transport is moved in vertical direction z direction...

Page 78: ...ownload 6 48 Operating Manual for the LS Series Scanner No 30001798 Rev No 1 5 2004 11 Note MTP Autofocus is aligned to the adapter i e all dimensions are oriented to the table In contrast HS and Well...

Page 79: ...e angle of incidence for the fluorescence excitation can be varied between 0 and 25 in steps of 0 1 via the software The geometry of the sample determines the angle of incidence The angle should be se...

Page 80: ...al incidence for image scan and 10 for autofocus Set Beamshift Set Beamshift may be a useful optimization parameter if your sample has only flat walls with a relatively low aspect ratio The excitation...

Page 81: ...rding to figure below to calculate the appropriate angles for your samples 25 0 6 5 Figure 6 9 Adjustable laser beam angle 0 1 increments High working distance 6 5 mm Set beam angle to 0 to avoid shad...

Page 82: ...in which all slides are selected see Slide Settings The Open dialog box The Open dialog box is the standard Windows file open dialog box It contains the following elements Look in combo box In the Loo...

Page 83: ...cessed will not be saved If a measurement parameter file with the same name already exists it will be overwritten The Save As dialog box The Save As dialog box is the standard Windows file Save As dia...

Page 84: ...omatically or not If the images should be stitched or not If the stitched image should be flipped or not The Filename can be either a standard name or an individual Filename Format AutoSave Stitching...

Page 85: ...g elements Select the Use standard name check box to enter the name in the standard name edit box The name gets part of the image filename Normally the standard image filename is grouped as follows St...

Page 86: ...2 row 2 The Process grid column displays check boxes for each slide defined All slides selected are processed during next measurement Select the check boxes of the corresponding slides to be processe...

Page 87: ...ition current date image counter if a barcode reader is installed and the barcode option is selected Excitation wavelength slide position current date image counter if no barcode reader is installed I...

Page 88: ...cally For example if Flip image up down is selected the result will be as follows When Flip image up down and Stitch resulting images are selected the result will be as follows Flip image left right F...

Page 89: ...nual for the LS Series Scanner No 30001798 Rev No 1 5 6 59 Rotate image Rotates image clockwise 180 For example if Rotate image is selected the result will be as follows When Rotate image and Stitch r...

Page 90: ...ved as 16bit TIF format Scanning of microplates or other slide adapter formats will open a lot of image windows in the Tecan Array Pro Software AutoSave is automatically enabled in Stacker Mode no use...

Page 91: ...efined for the following file types Images tif jpg Parameters lsp Adapter saf Temp all files tif jpg lsp saf autofocus images when AutoSave has not been selected see Files on page 6 60 Example After a...

Page 92: ...r pressing Start Prescan button are green those that will not be scanned are dark gray The selection can be changed in the Measurement dialog box Click on a slide to toggle its selection For Prescan o...

Page 93: ...35 Scan Mode S Single DS Dual Sequential DP Dual Parallel Scan Mode Gain Channel1 Gain Channel2 Laser Channel1 Filter Channel1 Laser Channel2 Filter Channel2 In this example three different scans are...

Page 94: ...ining filter slides Locking and unlocking the instrument for transportation purposes Calibrating the gain of PMTs Defining crosstalk Setting up and calibrating the stacker 6 4 2 Starting Scanner Shell...

Page 95: ...right Click the Help button to display the online Help for elements such as Buttons Menus Windows Click the Connect button to directly connect to an instrument Click the Measurement button to display...

Page 96: ...n dat cvs Gain calibration data is saved as GainCalibration dat files Gain calibration data is stored as a comma separated list cvs files For analysis these files can be loaded in Excel or another cal...

Page 97: ...arameter Slide adapter format saf SAF Gain calibration dat csv CalData Crosstalk dat CalData Temporary Files Temporary files needed for diagnostic and controlling purposes are stored in the directory...

Page 98: ...lect the Toolbar option to show hide the Scanner Shell software Toolbar When the Toolbar option is selected the Scanner Shell software Toolbar will be shown When the Toolbar option is cleared the Scan...

Page 99: ...uccessful Scanner Server Version V 2 20 No instrument connected Successful Scanner Server Version V 2 20 LS SERIES SCANNER LS 12902000004 V 3 00 08 03 IMAGING V1 00 08 01 MEAS IMAGE iV 1 10 Working in...

Page 100: ...nstrument menu select Edit Measurement Parameters or on the toolbar click the Edit Parameter button or press Ctrl F5 and the Measurement Parameter dialog box appears In the Measurement Parameter dialo...

Page 101: ...uments two filter slides can be loaded simultaneously Parameters and comments for one two filter slides channel 1 channel 2 must be set before starting measurement The first time is done by a service...

Page 102: ...slide contains 6 filter positions Position 1 is reserved for focus measurement Positions 2 5 are free for filter definition Position 6 is reserved Note Position 1 is always reserved for focus measurem...

Page 103: ...1 Pos 6 static text This static text corresponds to the filter positions on the filter slide loaded in filter slot 1 Pos 1 is reserved for focus measurement Pos 2 5 are free for filter definition Pos...

Page 104: ...static text corresponds to the filter positions on the filter slide loaded in filter slot 2 Pos 1 is reserved for focus measurement Pos 2 5 are free for filter definition Pos 6 is reserved Instrument...

Page 105: ...k Cancel to close the dialog box The Test Command dialog box is used in case of errors and for testing purposes Including but not limited to the following No or faulty communication between PC INSTRUM...

Page 106: ...rver can be simulated no instrument needs to be connected This option is used for training purposes For further information refer to the chapter 6 2 Tecan LS Scanner Interface description in this manu...

Page 107: ...Data from the PMTs becomes less accurate over the course of its life span so gain calibration should be repeated regularly After 100 days the user will be requested to start gain calibration again No...

Page 108: ...the Cy3 channel Typically the shorter wavelength dye into the longer wavelength dye s detection channel is much stronger In our example of Cy3 and Cy5 there is a significant contribution from Cy3 to C...

Page 109: ...st box All data selected entered in the Crosstalk dialog box is stored as a crosstalk factor record CrosstalkCor dat This can be added to K1 K2 data list box Clicking on an item in the list box displa...

Page 110: ...e entered Gain 2 edit box In the Gain 2 edit box PMT Gain channel 2 must be entered Next part of Crosstalk dialog box contains the following elements Channel 1 Laser combo box In Channel 1 Laser combo...

Page 111: ...lk factor record calculate the crosstalk correction factor and add a new item to the list box Change button Click the Change button to change the current selected crosstalk factor record in list box O...

Page 112: ...ion about Stacker Setup refer to the chapter 6 4 7 Stacker Setup Twister description in this manual In the Stacker Setup dialog box the user can choose between several options setting up the connected...

Page 113: ...ial slide adapter files For further information about creating and editing Slide Adapter Format files refer to the chapter Advanced Menu Edit SAF on page 6 85 description in this manual In the Open Sl...

Page 114: ...slide adapter format is displayed Slides group box In the Slide group box slide format data corresponding to selected files in File combo box are shown Number of columns Number of rows Area width Area...

Page 115: ...r and save adapter parameters the Slide Adapter Format dialog box must be displayed In the Advanced menu select Edit SAF and the Slide Adapter Format dialog box appears In the Slide Adapter Format dia...

Page 116: ...sions of individual areas are determined in the Area width controls Area height controls The overall dimensions of the scanned surface can be entered Left margin controls Upper margin controls Right m...

Page 117: ...ameters of a single scan area are represented in the Geometry Parameter Tab of the Measurement parameter dialog box of LSServer interface Close Click the Close button to save the entered slide data an...

Page 118: ...The Show Logfile option starts the standard Windows editor i e notepad and shows the LSServer log file When the Show Logfile option is selected the LSServer log file will be opened When the Show Logfi...

Page 119: ...The following modifications have to be performed 1 Set up the Twister Bottom Plate as described in the Twister Manual 2 Unscrew the instrument feet from bottom of the scanner add spacers to instrument...

Page 120: ...is connected using COM 2 because COM 1 is reserved for other instruments such as the Tecan LS SERIES SCANNER Clicking OK saves all entered data and closes the Port Setup dialog box The Port Setup dial...

Page 121: ...Back button can be used to navigate back to the previous window within a wizard In the first window of a wizard the Back button is not active Next button The Next button can be used to navigate forwar...

Page 122: ...hock absorber pad In the End Setting Transport Lock window the transport lock procedure is completed Once the instrument is locked no further measurement is possible To unlock instrument the Transport...

Page 123: ...ts Status Control bitmaps show the results of the operations The Position Transport window The Next button guides the user to the next screen of the wizard the Apply Absorber Pad window The Apply Abso...

Page 124: ...1 The End Setting Transport Lock window The End Setting Transport Lock window informs the user that the transportation lock has been set and instructs the user how to complete the process The End Sett...

Page 125: ...e user is asked to remove the shock absorber pad from the instrument In the Reset Transport Lock window lock status is removed and transports are initialized In the End Remove Transport Lock window th...

Page 126: ...w the user is instructed to remove the transportation shock absorber pad found in instrument The Remove Absorber Pad window The Next button guides the user to the next screen of the wizard the Reset T...

Page 127: ...be installed CAUTION BEFORE TRANSPORTATION Place the instrument carefully in the indicated orientation before fixing the transportation locks otherwise the instrument will become damaged The Reset Tra...

Page 128: ...lides Gain Calibration PMT 1 Put the calibration filter slide in filter slot 1 and the normal filter slide in filter slot 2 Gain Calibration PMT 2 Put the calibration filter slide in filter slot 2 and...

Page 129: ...rm that the special slides have been loaded and the user is guided to the next screen of the wizard The Prepare Gain Calibration window The Prepare Gain Calibration window In the Prepare Gain Calibrat...

Page 130: ...The Measurement progress bar indicates the progress of the ongoing process Stop button Click Stop to stop the measurement A message box is shown Click Cancel to continue the measurement Clicking Next...

Page 131: ...r button is clicked The LSDownload dialog box contains the following elements Instrument group box Connect instrument button Click the Connect instrument button to open the Setup Port dialog box The S...

Page 132: ...ins the following elements Save in combo box In the Save in combo box select the drive and the folder in which the download report should be saved Workspace list box The Workspace list box shows the f...

Page 133: ...P The following dialog box is displayed This is the standard Windows Print dialog box Click OK and the printing of the report of the current download is started Print Setup The Print Setup option in t...

Page 134: ...Preview option has the following buttons Button Description Print Selects the print option to print the report Next Page Displays the next page of the report Prev Page Displays the previous page of t...

Page 135: ...n is cleared the LSDownload software Status Bar will be hidden Instrument Menu Connect This option allows an instrument to be connected In the Instrument menu select Connect or in the Instrument group...

Page 136: ...n combo box In the Look in combo box the folder from which the Download files can be opened is selected Workspace list box The Workspace list box shows the files and folders selected in the Look in co...

Page 137: ...g box appears which recommends to stop all programs that are currently running The desired data will be sent to the connected instrument After the download has successfully finished a dialog box appea...

Page 138: ...can then be connected This option is only available if an instrument is already connected Help Menu Contents In the Help menu select Contents and the Online Help for LSServer Scanner Shell Download is...

Page 139: ...y external PC Image processing Integrated Software package Array Pro PC Delivered together with the instrument Operating system Windows 2000 Interface Serial interface RS 232 and high speed data acqui...

Page 140: ...be defined and added by the user Dichroic Beamsplitter Dual channel instruments only Two different beamsplitters automatically selected An additional beamsplitter can be implemented Pinholes 3 pinhol...

Page 141: ...Approvals DIN EN 61010 1ff UL 3101 1 C22 2 No 1010 1 Physical Outer dimensions Width 812 mm Height 355 mm Depth 566 mm 31 7 inches 13 9 inches 22 1 inches Weight approx 60 kg Environmental Ambient te...

Page 142: ...ONTROLS OR ADJUSTMENTS OR PERFORMANCE PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE 7 1 2 Optional Barcode Scanner The barcode laser scanner optional is for t...

Page 143: ...o 30001798 Rev No 1 5 7 5 7 1 3 Reproduction of labels placed on the instrument Labels placed on the rear front of the instrument Labels placed inside the instrument when housing is opened LASERSTRAHL...

Page 144: ......

Page 145: ...instrument The cleaning and maintenance procedures are important in order to prolong the instrument s life and to reduce the need for servicing This section contains the following procedures Liquid S...

Page 146: ...ompartment by inserting a screwdriver into the slot in the top of the cover and push the fuse holder out On Off Switch 1 ON 0 OFF Fuse compartment Main power socket Pull the fuse out and replace the d...

Page 147: ...ccess the ftp server 8 4 2 Working with pcAnywhere When pcAnywhere is activated for the first time the software asks you to register the software Please follow the step by step wizard registration ref...

Page 148: ...11 4 The fist time the software is started the following warning appears Click Yes and proceed with step 5 If this message isn t displayed continue to step 7 5 Create a new caller On the Callers tab...

Page 149: ...king with CITRIX web conferencing manager For online demo training and troubleshooting between Tecan and your company Tecan offers an ftp site It is accessible through http travel tecan ch Please down...

Page 150: ...t supplied the instrument may not be accepted by the servicing center or it may be held by the customs authorities 8 5 1 Disinfection Solutions If the laboratory has no specific disinfection procedure...

Page 151: ...urned 6 After the disinfection procedure has been performed ensure that the disinfection certificate is completed Note See Disinfection Certificate below for an example of the disinfection certificate...

Page 152: ...waste disposal Pollution degree 2 Method of Disposal Electronic waste Contaminated Waste infectious waste 8 8 1 Disposal of Packing Material According to Directive 94 62 EC on packaging and packaging...

Page 153: ...ng Material 8 8 Packing Material 8 8 Disposal of Instrument 8 8 Download File Opening 6 106 Dual channel instruments 2 3 F File Menu LS Download 6 102 File types Scanner Shell 6 66 Filename Format 6 5...

Page 154: ...Mode 6 47 N Non confocal measurements 2 3 O Optical Method 6 43 P Packing Material Disposal 8 8 Returning 8 8 Pinhole 6 38 Plate Move plate out after measurement 6 30 Plate and Filter Moving Scanner S...

Page 155: ...edure 6 2 Requirements 6 2 Stacker Check box 6 30 Port Setup 6 90 Setup 6 82 Start 6 11 Status bar Scanner Shell 6 65 T Test Commands 6 75 Toolbar Scanner Shell 6 65 Transport Lock 6 76 Transport Lock...

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