6. LSServer, Scanner Shell and Download
2004-11
Operating Manual for the LS Series Scanner No. 30001798 Rev. No. 1.5
6-41
Crosstalk
Crosstalk
group box
The
Crosstalk
group
box contains the following elements:
•
The
Crosstalk correction
check box
Select
Crosstalk correction
to select a correction factor
(= K-factor) from the Crosstalk list box.
Dual channel instruments, operated in Dual Parallel Scan Mode, are
corrected for the spectral crosstalk from the first dye into the second
detection channel by applying K-factors.
The
Crosstalk correction
check box is automatically available, if a Gain
Calibration file and a Crosstalk file are found for the current laser and filter
combination (selected in the Laser Channel 1 / Channel 2 and Filter
Channel 1 / Channel 2 combo box).
Otherwise Crosstalk elements are not enabled and no crosstalk correction
factor can be set.
The defined K-factors for the current laser / filter combination are displayed
in the Crosstalk list box. Clicking on a Crosstalk list item sets the K-factor
(values appearing in
K1 / K2
static text box), which will be used when
starting the next measurement, if the
Crosstalk correction
check box has
been selected. If the
Crosstalk correction
check box has not been selected
no correction is done.
•
The
K1
static text box displays the current selected crosstalk factor K1
(residual signal contribution from the first dye detected in the second
channel will be corrected).
•
The
K2
static text box displays the current selected crosstalk factor K2
(residual signal contribution from the second dye detected in the first
channel will be corrected).
•
In the
Crosstalk
list box all crosstalk factor records, corresponding to
selected laser / filter combination, are displayed:
Format
K1
=
Dye 1
,
Name
,
Date
Format
K2
=
Dye 2
,
Name
,
Date
For further information refer to the chapter 6.4.6 Service Menu -
Crosstalk…in this manual.
Note
In order to obtain reliable results it is strongly recommended to place
reference spots with one dye only onto your sample. These spots should
represent every dye of your experiment and should be used for QC
purposes.
