TAKARA PCR TP350 User Manual Download Page 2

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NOTICE

L18 Gradient Thermocycler

This product is licensed under U.S. Patent Nos. 5,525,300; 5,779,981; 6,054,263; 6,962,821, and

corresponding foreign counterparts thereto.

L45

Heated Cover

This product is covered by the claims of U.S. Patent 5,552,580, and their corresponding foreign

counterpart patent claims.

・

Thermal Cycler Dice

is a trademark of TAKARA BIO INC.

・

All marks are the property of their respective owners. Certain trademarks may not be registered

all jurisdictions.

Summary of Contents for PCR TP350

Page 1: ...IO INC Attention Users are recommended to carefully read the contents of this manual before operating the TaKaRa PCR Thermal Cycler DiceTM Touch Carefully observe all special Warnings and Cautions out...

Page 2: ...g foreign counterparts thereto L45 Heated Cover This product is covered by the claims of U S Patent 5 552 580 and their corresponding foreign counterpart patent claims Thermal Cycler DiceTM is a trade...

Page 3: ...nd Their Functions 11 Chapter 4 System Operation 13 1 Introduction 13 2 PCR Profile Creation 15 3 PCR File Manager 22 4 Manual Incubation 24 5 Configuration Tools 25 Chaptur 5 Diagnosis Functions 29 1...

Page 4: ...nsibilities Refer to the Warrantycard included with this product for detailed description of the Warranty conditions and period This product is designed to ultimate safety standards Nevertheless shoul...

Page 5: ...internal overheating that may result in physical burns or fire Avoid installing the system in locations subject to dust or strong vibrations Avoid installing in locations subject to direct sunlight a...

Page 6: ...ng the system Other precautions Observe the following precautions in order to prevent electric shocks and fires Avoid inserting metal objects flammable materials or other foreign objects into the vari...

Page 7: ...fires Grounding Mandatory Power cable Mise la Terre Obligatoire Cordon d alimentation Always ground the system to prevent electric shock from leakage current Burns High Temperatures Heat cover Heat bl...

Page 8: ...copied without the express permission of the copyright holder Copyright holder TAKARA BIO INC Printed in Japan Contact TAKARA BIO INC TAKARA BIO INC SETA3 4 1 OTSU SHIGA 520 2193 JAPAN Tel 81 77 543...

Page 9: ...g system configured and optimized for industry standard 0 2ml individual or strip tubes domed or flat capped or 96 well plates Applications of thermal gradient technology gradient of between 0 25 12 s...

Page 10: ...of heating and cooling between 0 1 sec 2 5 sec during a run Manual Control mode enables the user to set the block to a specific temperature quickly without creating a thermal profile This function is...

Page 11: ...ar equipment that generate noise caused by electrical motors and transformers 6 Choose a flat location capable of withstanding the design weight of the system 7 The installation environment must be wi...

Page 12: ...1 Thoroughly clean the system and make sure that there are no tubes or plates remaining in the sample block 2 Avoid any shock during movement that can affect the operation of this highly sophisticated...

Page 13: ...ting fingers as the Top Heater interior of the unit reaches very high temperature up to 115 in operation Touch Panel 7 inch vivid color touch screen controls the program at your tip of your finger or...

Page 14: ...which sample tubes and plates are placed Caution Do not touch the block as it reaches temperatures up to 99 Power Switch The Power Switch is main switch to put on or shut off the power Power Cable Con...

Page 15: ...will display the logo and take a few moments to initiate the software Caution Please do not touch the Touch panel and shut down the system until the main menu appear on the panel screen That is the ca...

Page 16: ...vide access the following features PCR Program Editor is used to create a new PCR thermal profile and initiate its execution PCR File Manager is used to load and edit PCR program files Manual Incubati...

Page 17: ...a stored program on the device Add Step Add a step file to specify the location of the displayed profile Add to end of Profile Add a step to end of the displayed profile Insert after X Add a step clo...

Page 18: ...ck hat icon worn Wizard under the screen and open the default profile Change a block temperature to click a temperature column Change a reaction time to click a time column Change a number of cycles t...

Page 19: ...rresponding check boxes Each of the steps will be executed in the order they are shown arranged down the screen with the denaturation Anneal and Extension if enabled steps being cycled After the ampli...

Page 20: ...ple hold at given temperature for a set time It may be performed either with or without a gradient across the block If you wish to hold the temperature infinity you may touch the Infinite button Ramp...

Page 21: ...gressively increased or decreased from an initial value to a final value over a defined number of steps Long Range allows the hold time of a given step within a cycling event to be progressively incre...

Page 22: ...kbox option is selected default then you will be prompted with a screen asking if you wish to wait for the top heater or reach target temperature before commencing the run If you select to wait for th...

Page 23: ...use and stop using right icons on the Run Screen On run completion the post run report screen will show and if enabled an audible alarm will sound The alarm may be muted by pressing the mute alarm but...

Page 24: ...around plural target files by a stylus and then select Copy icon on the lower side of screen Paste Appear a Paste icon when select a Copy icon on the lower side of screen Paste copied files for other...

Page 25: ...er s Manual PostRunReport The post run report can be saved as a PostRunReport text file on the PCR File Management maximum recent 10 files Specification is described content of a PostRunReport file v...

Page 26: ...ight sides of the block with a roughly linear thermal gradient presented between the two outer well sides and the predicted well temperatures can be shown by clicking the View Gradient Temp link Top H...

Page 27: ...er recalibration for the life of the machine The recalibration procedure is not covered in this user manual If it is required contact the support line service of Takara Bio The thermal calibration is...

Page 28: ...the users area you are able to select an existing user or create or delete a user profile When a user is selected thermal profiles will be loaded or saved to a directory specific to that user Logging...

Page 29: ...Most manufacturers thermal cyclers purely use the block temperature for timing Accelerated Thermal Equilibration If this feature is selected the temperature model will use overshoot and undershoot to...

Page 30: ...the software which resides on the Takara Thermal cycler DiceTM Touch The upgrade file you receive from our support team should be placed on the main root directory of your USB memory stick You may the...

Page 31: ...t at room temperature prior to commencing test The Diagnosis function performs the following three tests 1 Cycle Test From the home screen the PCR File Manager icon will bring to the experiment page Y...

Page 32: ...efore confirming commencement and then click Start Run The Program is started The below report is described to the end of Cycle Test program Run Started Run completed and Run duration is appeared The...

Page 33: ...st 3p file to preset program in Dice and appear the specification of this program at the right side of PCR File Manager Touch a Edit File icon show the content of program Program details 98 for 1min 2...

Page 34: ...bed to the end of Up Down Test program Run Started Run completed and Run duration is appeared The report is saved a PostRunReport file You checked that the run durations less than the Standard Value T...

Page 35: ...Select the Ramp Rate Test t3p file to preset program in Dice and click the checkmark Touch a Edit File icon show the content of program Program details 25 for 30sec 95 for 30sec 40 for 30sec 1 cycle...

Page 36: ...P350 User s Manual The Program is started The below report is described to the end of Ramp Rate Test program Click the lower right check then the Configuration is selected from the home screen v2 01 3...

Page 37: ...on the Calibration Wizard screen The Max Cool rate and Max Heat rate is displayed on the System Massage Reference Value Max Cool is more than 2 5 sec Max Heat is more than 3 0 sec After ramp rate is c...

Page 38: ...e Top Heater module Need to replace the Top Heater Module PCB fault Need to replace the Main Module Touch screen does not work but when a mouse is plugged in it works and the touch screen cannot be re...

Page 39: ...power switch and take off the power cord for safety 2 Warn to spill neither water nor a reagent to mechanism parts and electric parts Moreover please place neither detergent nor medicine on equipment...

Page 40: ...e When a heated top heater becomes by power failure below in a predetermined temperature resume operation after warming a heated top heater to an appropriate temperature The program will be ended and...

Page 41: ...l Memory 256MB enough for 10 000 saved profiles USB Flash memory is enable to use Heated Top Heater range Controllable 60 115 Internal Interface Embedded graphical controller with 7 widescreen touch s...

Page 42: ...tylus Touch pen 1 4 Warranty sheet 1 5 TaKaRa Supplied PCR Tubes trial set Single Tube 0 2 ml Hi Tube Dome Cap Single Tube Cat NJ200 100 8 Tube Cap 0 2 ml Hi 8 Tube 8 Tube Cat NJ300 12 0 2 ml Hi 8 Fla...

Page 43: ...8 Cap NJ300 0 2 ml Hi 8 Tube 8 Tube 125 NJ301 0 2 ml Hi 8 Dome Cap 125 NJ302 0 2 ml Hi 8 Flat Cap 8 cap 125 NJ600 0 2 ml 8 strip tube individual Flat Caps 120 0 2 ml Plate Cap NJ710 96 well snap plat...

Page 44: ...athogenicity none possibly yes Toxicity none possibly yes Radioactive substances not used used nuclide 2 System contamination status no contamination decontamination possibly contaminated This is to c...

Page 45: ..._________________________________________________________________________ ______________________________________________________________________________________________________________________________...

Page 46: ...SETA3 4 1 OTSU SHIGA 520 2193 URL http www takara bio com 2015 03 09...

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