TP350 User’s Manual
２－２．
Configure a new profile using Wizard Mode
The TaKaRa PCR Thermal Cycler Dice
TM
Touch
software includes a Wizard
utility which enables the user to configure easy to moderate complexity profiles in
just moments. To activate select ‘Wizard’ from the PCR or One Step RT-PCR
start-up screen.
The Wizard screen displays each of the steps which occur in a typical range of
profiles. At a usual there are 3 steps, Preheat and then denaturation and anneal
which are cycled. You may enable up to a further 3 steps (cDNA, Extension & post
run hold) by clicking on the corresponding check boxes. Each of the steps will be
executed in the order they are shown arranged down the screen with the
denaturation, Anneal and Extension (if enabled) steps being cycled. After the
amplification run has been completed the temperature may be held at a defined
temperature until the user disables the unit.
‘Hold on Completion’ is checked to hold at 8
℃
(default) that amplified samples
keep cooling.
To adjust any of the parameters click on the corresponding time or temperature
boxes and keypad will be presented allowing entering of new numbers. The
number of cycles can also be adjusted between 1 (single execution, no loops) and
99 cycles.
【
Range of values
】
Block temperature
：
4
℃
～
99
℃
、
Time
：
1sec
～
9hour
、
Number of cycles
：
1
～
99Cycles
、
Top heater temperature
：
60
～
115
℃
v.2.01
17
