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DOCUMENT #28940
4
Flag
1.1.4
The Tip Stripping Arm
RoboSep™ disposable tips are returned to the tip rack after each use. This eliminates the need for a waste tip area on the instrument
and facilitates easy clean-up after a run by allowing the entire tip box to be disposed of appropriately. Figure 7 illustrates the automated
pipette tip removal process.
1.1.5
The Hydraulic System
The hydraulic system uses sterile deionized water driven by a positive displacement piston pump. The pipette handling tip head is
connected to the reservoir bottle containing the sterile deionized water via the pump. The only visible parts of this system are the water
bottle and the tubing running from the console to the robotic arm. The hydraulic fluid does not make contact with the user’s samples or
reagents, and because it is deionized water, it is less damaging to the piston seal than salt solutions or buffers. The RoboSep™ pump
therefore has a substantially longer operating life than the seals on syringe pumps used in other instruments and does not require any
user maintenance. The use of water as a hydraulic fluid instead of air allows for accurate high-speed dispensing: the water effectively
acts as an incompressible extension of the pump piston, allowing the system to operate with a dead air volume similar to that of a
manual pipettor.
1.1.6
Homing the Carousel and Robotic Arm
RoboSep™ tracks the position of the robotic arm and carousel relative to physical markers called homing flags. A home position is
registered by the computer when a homing flag trips an optical sensor. The carousel homing flag is readily visible as a projection on the
bottom plate (Figure 8). This projection trips an optical sensor which can be seen on the front inside wall of the bowl when the carousel
is removed. The robotic arm homing flags and sensors are not visible. Homing of the carousel and robotic arm is performed
automatically at start-up, at the beginning of each cell separation experiment and after the instrument has been paused during a run.
The robotic arm home positions are detected again periodically throughout a run.
To ensure correct operation of the instrument, it is essential that no
mobile parts be moved or touched during an experiment.
Homing is the
only mechanism by which RoboSep™ determines the location of items on
the carousel; any disruption of the carousel or the arm during operation can
cause pipetting actions to fail. In order to prevent such disruptions,
RoboSep™ operates with a transparent lid over the arm and carousel. If the
lid is opened during an experiment, the instrument will pause once the
current action is complete. Once the lid is closed, RoboSep™ will
automatically execute a homing sequence and resume the experiment.
Figure 7 – Disposable 5 mL pipette tip being
replaced in the tip rack. The robotic arm places
the used tip part way into the rack (A). The tip
stripping arm swings out to engage the tip head
just above the tip (B). The robotic arm then
moves up and the tip falls into the tip rack (C).
Figure 8 – The carousel home flag is visible as a projection
from the bottom plate in this schematic diagram
Summary of Contents for RoboSep 20000
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