STEMCELL RoboSep 20000, Technical Manual

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THIS PRODUCT IS MANUFACTURED AND CONTROLLED UNDER A QUALITY MANAGEMENT SYSTEM CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
TOLL FREE PHONE 1 800 667 0322
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PHONE +1 604 877 0713 VERSION 2.4.0
INFO@STEMCELL.COM
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TECHSUPPORT@STEMCELL.COM
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FOR GLOBAL CONTACT DETAILS VISIT WWW.STEMCELL.COM DOCUMENT #28940
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6 TROUBLESHOOTING
RoboSep™ presents error messages to help diagnose some types of problems that may occur during use. Please record the message
before calling Technical Support. The following tables address other potential issues that are not flagged as errors.
PROBLEM POTENTIAL CAUSE SOLUTION
RoboSep™ screen does not turn on. No sound,
movement, or sign of activity within 30 seconds
of attempting to power up the instrument.
Faulty fuse.
Check that the fuses are correctly installed in
described in Section 5.6.
No power to outlet.
Check that the outlet is active by connecting a
known working device such as a lamp.
Operating system boots up but RoboSep™ fails
to fully initialize.
Power switch not left on long enough. It takes up
to 10 seconds for the electronics to fully reset
after the power switch has been turned off. After shutdown, turn off the power switch and
count to 10 before turning the power back on
again.
RoboSep™ won’t resume a paused run.
Lid up. RoboSep™ will not run if the lid is in the
raised position.
Lower the lid and press Resume on screen.
RoboSep™ won’t shut down because the basic
shutdown protocol won’t load. Previous run not complete or needs to be
unloaded. Let run finish. Press unload button on the
Sample Processing screen.
Reagents left in tips.
RoboSep™ leaves a residual volume in tips
when pipetting small volumes.
This is a normal operation and is required to
meet the accuracy requirements for low volume
reagent addition. The residual volume is 20 µL
or less.
Missed tip pick-up.
Tip rack not placed properly on the carousel.
Make sure that the tip rack is seated properly by
pushing down firmly on the outside of the
exposed portion.
Robotic arm or carousel was bumped during
operation.
Only operate with the lid down.
If the above measures fail to solve the issue,
contact Technical Support.
Tip head spits hydraulic fluid.
Hydraulic fluid line contamination.
Fill hydraulic fluid bottle with 70% isopropanol or
70% ethanol and prime >4 times. Leave alcohol
in line for >1 hour (longer times preferred), prime
>4 times with sterile deionized water. If problem
persists, contact Technical Support.
Damage to fluid line. Contact Technical Support.
Tips not well aligned with tubes. Positional calibration is required. Contact Technical Support.
Tip rack separates at the snap joint when
removing cap.
Excess force applied at this joint by mistake. Ensure only the top cap is removed.
Missed tip stripping action.
Tip stripping arm not moving.
Arm is out of position. Power down the
instrument, open the lid, and pull the tip stripping
arm out. Restart RoboSep™.
Tip stripping arm misaligned. Contact Technical Support.
Priming step missed or incomplete: No water
ejected from the tip head prior to run.
Connections in hydraulic system are leaking air.
Make sure the connection between the hydraulic
fluid bottle and tubing is seated correctly by
disconnecting and reconnecting firmly.
Hydraulic fluid bottle is empty. Refill bottle.
Can’t load protocol in Protocol Editor. File is read-only. Change file property.
Custom protocol does not appear in protocol
database. File label is not unique. Create unique label for protocol using Protocol
Editor (see Section 3.3).