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FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
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DOCUMENT #28940
18
Source:
This is the container (tube, reagent vial, or buffer bottle) from which the pipette will aspirate. If a reagent vial is specified as the
source, a 1 mL pipette tip is used automatically. If a sample, separation, or -negative fraction tube is the source, then the 5 mL tip
reserved for cell handling is used. If the source is the buffer bottle then the 5 mL tip reserved for buffer handling is used. The source is
chosen from a pull-down list which is sorted by relative quadrant number. The source tube should be in Quadrant 1 in most cases,
since Quadrant 1 is the start quadrant for all protocols. Tubes in Quadrant 2 are only accessed in protocols requiring two quadrants
(e.g. some negative selection protocols).
Destination:
This is the container into which the pipette will dispense. The destination container is also selected via pull-down menu.
The list is sorted by relative quadrant number. A protocol with a source in Quadrant 1 and a destination in Quadrant 2 will occupy two
quadrants on the carousel. If this protocol is loaded in Quadrant 3, the destination will be in Quadrant 4.
Free-Air Dispense:
When this check box is enabled, RoboSep™ dispenses the fluid that it is transporting just below the upper lip of
the destination container and above the liquid in the container. This option is useful where contact with the contents of the destination
tube is undesirable (e.g. preventing contamination of the designated buffer tip when adding buffer to the sample tube.).
Note: This option is not recommended for transfer of small volumes (e.g. addition of reagents).
Volume:
For Transport commands, the user can specify whether RoboSep™ is to transport an absolute volume (in µL) or a volume
relative to the initial sample volume (e.g. to have RoboSep™ add 100 µL of reagent per mL of sample volume, program the addition of
a relative volume with a proportion of 0.10).
Incubation Time:
This parameter specifies the length of time, in seconds, for reagent and magnetic separation incubations.
Summary of Contents for RoboSep 20000
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