STEMCELL RoboSep 20000, Technical Manual

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THIS PRODUCT IS MANUFACTURED AND CONTROLLED UNDER A QUALITY MANAGEMENT SYSTEM CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
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1.2.5 Priming the System
RoboSep™ performs a priming sequence at the beginning of every cell separation protocol to minimize the effect of air bubbles that
naturally accumulate in the hydraulic system. However, when RoboSep™ is used for the first time, or if the hydraulic fluid bottle runs
empty, it is necessary to run a separate priming protocol by following the steps below:
1. Fill the hydraulic fluid bottle with 250 mL of sterile deionized water and connect the bottle to the hydraulic system tubing
in the compartment located at the right of the instrument (Figure 9).
2. If the instrument is turned off, switch it on and wait until the end of the start-up sequence as described in section 1.2.4.
3. Press the “Instrument Tasks” tab, select “Basic Prime” under the “Maintenance” tab and then press the green “Load”
button.
4. Place a 50 mL tube in the appropriate slot of the carousel as indicated on the screen.
5. Press “Run.”
If no water is expelled from the tip head into the waste tube by the end of the priming sequence, there could be a problem with the
connections at the hydraulic fluid bottle (see Section 6 – Troubleshooting).
1.3 RUNNING ROBOSEP™: QUICK START INSTRUCTIONS
Note: Before using RoboSep™, ensure that the hydraulic fluid bottle is full (250 mL of sterile deionized water).
The following is a brief quick start guide to using the RoboSep™ fully automated cell separator; detailed instructions on running
RoboSep™ are provided in Section 2 of this manual. To quickly execute a run on RoboSep™:
1. Power up RoboSep™ and wait for initialization to complete.
2. Follow the instructions on the message bar at the top of the screen. Press “Select Protocol” to bring up the list of
available protocols (see Section 3.1.2 to customize the protocol list). Choose the desired protocol from the list and then
enter the sample volume. Repeat this process until all the desired protocols (up to four) are selected.
3. Press “Load” to begin the loading sequence. Lift the lid and load the indicated carousel quadrant(s) with the items listed
on the left of the touchscreen. Press “Loaded” once each quadrant is ready. When all quadrants are loaded, close the
lid.
4. Press “Run” and confirm you are ready for the instrument to start. RoboSep™ will now complete the selected protocols
unless it is paused or halted. The time remaining until completion is displayed on the screen.
5. Once the run is complete, press “Unload,” open the lid, and collect the cells of interest. For positive selection, the cells of
interest are in the magnet tube. For negative selection, the cells of interest are in the 14 mL sample tube of the second
quadrant (if using 2 quadrant negative selection), or the 50 mL negative fraction tube of the first quadrant (if using 1
quadrant negative selection).
6. Unload the rest of the carousel and store the reagents appropriately. Discard the tip boxes containing the used tips in the
appropriate waste container.
The instrument is now ready for another run. If no more runs are planned for the day, or if a spill occurred, clean the instrument as
detailed in Section 5 – Care and Maintenance. To conserve energy, turn off the instrument at the end of the work day.
Note: RoboSep™ protocols may differ from the standard manual separation protocols because they have been optimized for an
automated system. Unless otherwise noted, all RoboSep™ procedures have been optimized for use at room temperature (15 - 25°C),
regardless of the temperature recommended for the manual procedure.