STEMCELL RoboSep 20000, Technical Manual

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THIS PRODUCT IS MANUFACTURED AND CONTROLLED UNDER A QUALITY MANAGEMENT SYSTEM CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
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9
Minimum reagent
volume required
Scroll button for
reagent list
Waste Tube
Separation Tube
Negative Fraction /
Lysis Buffer Tube
Sample Tube
Tip Rack
EasySep™
Reagent Vials
2.2.5 Loading the Carousel
Once all the desired protocols have been selected and the cell samples have been prepared, the carousel can be loaded:
1. Press the “Load” button. If the same protocol has been
selected in more than one quadrant, a pop-up window will
open to give you the option of using a single set of reagents
for all identical separations (Figure 14). Alternatively, one set
of reagents may be used for each individual separation.
2. An image of the carousel will appear (Figure 15). The active
quadrant number(s) is displayed at the bottom right of the
window. At the top center of the window, the name of the
protocol assigned to this quadrant is also displayed. On the
left hand side of the window is a list of all the reagents and
resources that need to be loaded for the current separation
(see Section 2.2.4 for suitable tubes). The list starts with
items near the center of the carousel and ends with items
near the rim to facilitate aseptic loading. The user can move
up and down the list by pressing the arrows located above
and below.
3. Touch the first item on the list. The item will be highlighted in
green and its location on the carousel indicated by a green
circle. Turn the carousel so that the indicated quadrant is in
a convenient position and place the item in the indicated
location. Continue down the list, loading each item in turn.
4. Load reagents. The minimum reagent volume required for a given cell separation procedure is displayed below the name
of each reagent. Verify that your vial has the minimum required volume. To assist in proper placement, the reagent vial
labels have symbols that correspond to symbols on the carousel and on the touchscreen. For recording reagent lot
numbers and sample IDs, first select the item on the list and then select the grey box under the “Reagent Lot ID”
heading. Alternatively, STEMCELL offers a barcode reader (Catalog #20501) that can scan barcodes on reagent vials
and sample tubes to record reagent lot # and sample IDs (see Section 2.2.6).
Important Notes:
x
RoboSep™ uses EasySep™ reagents in 1.5 mL vials from Sarstedt. Use of any other vial will cause RoboSep™
separations to fail.
x
Certain negative selection protocols use D magnetic particles that can be pulled to the side of the sample tube by
the external magnetic field of “The Big Easy” EasySep™ Magnet. While this does not affect performance, it can be
prevented by inserting a magnet shield between the magnet and the sample tube.
Figure 14 – One set of reagents can be used for multiple identical
separations
Figure 15 – Carousel layout. The locations
of the items listed on the left side of the
screen are indicated with a green circle
when selected. In this example, “Separation
Tube” is highlighted in green on the left, and
the location of the tube is indicated with a
green circle in Quadrant 1.