5. Start the solvent flow and sample injection.
Set the Starting Conditions
1. In the Tune Method Editor, make sure that the correct
Scan Type
and appropriate
compound parameters are selected.
2. Type
30
in the
Ion Source Gas 1 (GS1)
field.
3. In the Curtain Gas (CUR) field, type the value appropriate to the mass spectrometer.
Table C-1 CUR Parameter Values
Mass Spectrometer
Starting Value
SCIEX 3200
20
4. Type
1
in the
Nebulizer Current (NC)
field.
5. On the Compound tab, in the
Declustering potential (DP)
field, type
100
.
6. Start acquisition.
Optimize Source and Gas Parameters
1. Adjust ion source gas 1 in increments of five to achieve the best signal or signal-to-noise
ratio.
2. Increase the flow rate of the gas for the Curtain Gas interface until the signal starts to
decrease.
Note:
To prevent contamination, use the highest possible value for the flow rate of the gas
for the Curtain Gas interface that does not sacrifice sensitivity. Do not set the flow rate lower
than the values in the table:
. This helps to prevent penetration of the flow of the
gas for the Curtain Gas interface, which can produce a noisy signal; prevent contamination
of the aperture; and increase the overall signal-to-noise ratio.
Table C-2 CUR Parameter Values
Mass Spectrometer
Starting Value
SCIEX 3200 systems
20
Ion Source Optimization
System User Guide
RUO-IDV-05-6475-C
SCIEX 3200 Systems
93/114
