Qiagen PyroMark Q96 ID, User Manual

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Glossary
8-4 PyroMark Q96 ID User Manual 01/2016
Comments and suggestions
b) Nonspecific peaks
Sequence signals from the self-annealed
sequencing primer and/or biotinylated PCR
primer and DNA template or nonspecific
annealing of the sequencing primer.
Run DNA template, sequencing primer, and
biotinylated PCR primer in individual wells. If
significant peaks appear, consider redesigning
the PCR and/or sequencing primer(s).
Contaminated nucleotides.
Background of contaminating sequence from
PCR. Consider redesigning sequencing primer.
Contact QIAGEN Technical Services if this
problem persists.
c) Low signal Too little template.
Reagents incorrectly stored or dissolved.
Too much salt in the sample.
Follow sample preparation instructions carefully,
see Section 5.4.
Dirty lens array. Clean the lens array, see Section
7.3.
d) High baseline noise Contaminated nucleotides or background of
contaminating sequence from PCR.
Contact QIAGEN Technical Services if this
persists.