Qiagen PyroMark Q96 ID, User Manual

Page: 42 / 126

Operating Procedures
5-10 PyroMark Q96 ID User Manual 01/2016
Procedure:
1. Ensure that the PyroMark Q96 Vacuum Workstation has
been assembled correctly and securely (see “Assembly of
PyroMark Q96 Vacuum Workstation”, page 5-7). The
mains plug should be easily accessible in case the
vacuum pump needs to be disconnected quickly from the
mains power.
Note : Perform the function test to ensure that the filter
probes are working properly (see Section 7.8.1). All
probes should be replaced after preparation of
approximately 100 plates, or when needed.
Note : Check and empty the waste container, if necessary
(see Section 5.7.5).
2. Fill five separate troughs (supplied with the PyroMark
Q96 Vacuum Workstation) as follows:

Approximately 110 ml ethanol (70%) (1)

Approximately 90 ml Denaturation Solution (2)

Approximately 110 ml Wash Buffer (3)

Approximately 110 ml high-purity water (4)

Approximately 180 ml high-purity water (Parking
Position, “P”).
A suggested setup is shown on the next page. Refill the
troughs to these levels whenever necessary.
The total volume that can be added to each trough is
180 ml. There is a mark on each trough indicating 90 ml.