Qiagen PyroMark Q96 ID, User Manual

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Glossary
9-2 PyroMark Q96 ID User Manual 01/2016
Term Description
IUPAC
International Union of Pure and Applied Chemistry. An
organization providing recommendations on organic and
biochemical nomenclature, symbols, terminology, etc.
IUPAC codes
A = Adenine K = T or G
C = Cytosine W = T or A
G = Guanine S = C or G
T = Thymine B = C, T, or G (not A)
R = Purine (A or G) D = A, T, or G (not C)
Y = Pyrimidine (C or T) H = A, T, or C (not G)
M = C or A V = A, C, or G (not T)
N = Any base (A, C, G, or T)
Out of phase When one of the alleles is sequenced ahead of the other.
Polymorphism Genetic variations, broadly encompassing any of the many
types of variations in DNA sequences that are found within
a given population.
Presequencing
signal
As the substrate is dispensed into a well (indicated by an
“S”), contaminants (such as ATP or PPi) will be converted to
light. Too high a substrate peak indicates that high levels
of a contaminant might be present in, for example,
buffers.
Pyrogram
The graph resulting from a sequencing reaction performed
using Pyrosequencing technology. Each incorporated
nucleotide is shown as a peak in Pyrogram.