Qiagen PyroMark Q96 ID, User Manual

Page: 80 / 126

Glossary
8-2 PyroMark Q96 ID User Manual 01/2016
Comments and suggestions
b) Results only in some
wells of the plate
Insufficient sample amounts. Follow sample
preparation instructions carefully (see Section
5.4).
Wells marked in the software do not correspond
to the sample placement in the PyroMark Q96
Plate Low. Check the plate was loaded according
to the information under the “Sample” tab in the
“Run Setup” dialog.
Insufficient enzyme or substrate for all the wells.
Check the reagent cartridge was filled according
to the “Volume Information” under the “Run Info”
tab.
8.2 Sequence errors
Comments and suggestions
a) Some peaks
appear, but the
sequence stops
Ensure that all nucleotide chambers in the reagent
cartridge contain nucleotides. Check the reagent
cartridge for blockages and rinse or replace it, as
necessary.
Check the sample and sample preparation.
Contaminated samples will lead to an unusually
high consumption of substrate at substrate
addition, giving a high presequencing signal and
no light produced at nucleotide dispensation.