PerkinElmer FLEXAR SQ 300 MS, User Manual

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Starting Chromera. 33
Running Auto Tune Using the Built-in Syringe Pump
Running Auto tune using the built-in syringe pump is very similar to running Auto Tune using the
Calibration vials except that the syringe parameters and flow rate must be input as demonstrated in the
following screen:
1. Open Auto Tune and select Syringe from the Analyte Source drop-down menu.
Make sure the syringe is loaded with desired calibrant and that the peek line extends from the
syringe tip and connects to the probe sprayer with a peek finger tight connector.
2. Click the Sample Prime button.
The Tune Setup dialog displays.
3. Type in the
System Name
and the
User Name
.
4. In the
Syringe Pump
section, select the
Make
of the syringe from the drop-down menu.
When using a 500 uL Hamilton Syringe select
Custom - user entered
in the drop-down and the
set the syringe inner diameter to 3.26 mm and the flow rate to 15 µl/min.
5. Click the Start Pumping button and wait until the mid-range mass shown in the scan window has
stabilized just as you did before when using the calibration vial.
6. Run Auto Tune and Check Tune as described earlier in this section.