13
UNO-TS. UNO-TS software is available for purchase upon request. UNO-TS includes a
Fine Gauge Thermocouple. UNO-TS allows logging temperature data, storing the data in
computer memory as well as performing calibrations in the lab. Additionally, the Fine
Gauge Thermocouple allows operation in Sample Feedback mode.
Equipment Required (Not supplied)
1.
CO
2
gas supply (100%)
2.
Compressed Air tank (or a compressed air line). If no air supply is available in the lab
please order air pump OKO-AP.
3.
Alternatively to 100% CO2 + air UNO can be operated with a pre-mixed 5% CO
2
gas
supply.
8
UNO-COMBINED-CONTROLLER. Overview
UNO-COMBINED-CONTROLLER is at the same time a temperature and a gas controller. It is
designed to maintain all the required thermal conditions for live cell imaging and is ideal for
time-lapse imaging applications.
Temperature Regulation
UNO-COMBINED-CONTROLLER controls the sample temperature by regulating the
temperature of the base and of the transparent heated lid of the incubating chamber.
Embedded thermistors read the temperature of the incubating chamber’s base, chamber’s lid
and humidifier module. A unique algorithm maintains these temperatures constant. A careful
calibration (See Table 1) performed in our laboratories guarantees that sample temperature is
maintained at the desired value by selecting the Standard Mode (See paragraph 13.5.1)
working modality. An external Fine Gauge Thermocouple (i.e. a thin flexible green wire
temperature sensor (optional) can be used to read the temperature of a reference well to
calibrate the chamber or to check that sample temperature is at the desired value when using
factory default configuration. This optional feature offers higher accuracy of temperature
control. Simply place the thermocouple into the reference well and hold it in place with some
adhesive tape (See paragraph 13.5). The chambers (‘Incubators’) accept different plate
adapters fitting many kinds of plastic supports, such as 35mm Petri Dishes, chambered slides,
simple glass slides and multi-well plates. (See Table 1). Please contact Okolab if you need
assistance selecting the correct incubating chamber fitting your microscope stage brand/model
and sample adapters.
CO
2
Regulation
CO
2
is mixed with air in the control unit and is continuously fed into the incubating chamber.
You can also use pre-mixed 5% CO
2,
connecting the pre-mixed
gas supply directly to the
Control Unit, to the input labeled ‘Air In’.
