NEW BRUNSWICK SCIENTIFIC BIOFLO III Manual To Operations Download Page 7

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Summary of Contents for BIOFLO III

Page 1: ...TORY SCALE FERMENTOR MANUAL NO M1226 0050 N NEW BRUNSWICK SCIENTIFIC CO INC BOX 4005 44 TALMADGE ROAD EDISON NJ 08818 4005 732 287 1200 TELEPHONE 800 631 5417 FAX 732 287 4222 TELEX 4753012 NBSCO INTE...

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Page 3: ...or replacing the instrument part thereof which shall within one year after date of shipment prove to be defective after our examination This warranty does not extend to any NBS products which have bee...

Page 4: ...UIRED PLEASE CALL OUR SERVICE DEPARTMENT AT 1 800 237 2298 PLEASE DO NOT RETURN ANY EQUIPMENT FOR SERVICE WITHOUT A RETURN AUTHORIZATION AND NUMBER WHICH CAN BE OBTAINED FROM OUR SERVICE DEPARTMENT TH...

Page 5: ...ervice Connections 2 5 Recorder Connections CHAPTER 3 PREPARATION AND OPERATION 3 1 Cleaning of Vessel 3 2 Vessel Assembly 3 3 Preparation Before Sterilization 3 4 Sterilization Procedure 3 5 pH Probe...

Page 6: ...4 8 Electrostatic Device ESD Warning 4 9 ESD Precautions APPENDICES I BioFlo III Operational Tips II Communication Protocols for NBS RS 232 422 DRAWING LIST...

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Page 8: ...Four sterilizable polypropylene compression ports are provided in the glass wall for the addition of antifoam and nutrients as well as for vessel overflow in continuous culture studies Ports are prov...

Page 9: ...M1226 0050 2...

Page 10: ...eadplate The controller operates the antifoam addition pump that adds chemical defoamer through the port in the wall of the vessel Fig 1 Sensitivity and time adjustments are made on potentiometers loc...

Page 11: ...e precise means of indicating flow rates in fluid systems Their design is based on a variable area principle BioFlo III is equipped with a 0 2 to 4 0 SLPM flowmeter NOTE 5L Unit uses an interchangeabl...

Page 12: ...itch to raise or lower the set point of the parameter selected Also used during calibration of pH and DO Selector Switch to select pH DO agitation temperature or nutrient readings to be displayed Mode...

Page 13: ...he bottom switch position AUTO is for automatic pump operation The center position is OFF External nutrient pump control Fig 7 The 6 lug terminal strip allows for external control of the nutrient pump...

Page 14: ...agitation switches to their off position 4 Check the specification plate on the rear of the unit and plug the line cord into a suitable electrical outlet 2 4 SERVICE CONNECTIONS Water 20 max PSIG 50 m...

Page 15: ...the Headplate Fig 9 2 Insert the sparger tube in the sparger port Fig 10 3 Insert the harvest tube in the harvest port Fig 11 Working From the Top of the Headplate 4 Insert the thermowell tube into t...

Page 16: ...t be free of glycerin 3 3 PREPARATION BEFORE STERILIZATION Before sterilization perform the following procedure 1 Fill vessel with water to its working volume 2 Place the vessel assembly on the three...

Page 17: ...riod of time 12 Check that the machine functions properly 13 Pull out the pH probe from the pH port and calibrate it see Section 3 6 3 4 STERILIZATION PROCEDURE 1 Remove the motor from the top of the...

Page 18: ...T MUST BE VENTED AT ALL TIMES DURING STERILIZATION OF THE GLASS REACTOR VESSEL CONTENTS AND ELECTRODES IT IS IMPORTANT THAT THE PRESSURE BUILT UP IN THE AUTOCLAVE IS RELEASED ONLY WHEN TEMPERATURE HAS...

Page 19: ...ode to probe cable Turn the agitation switch OFF Turn ON power switch NOTE The pH measuring system is calibrated using two buffer solutions of a known pH 1 Set the selector switch to pH Set the mode s...

Page 20: ...parge Nitrogen into the vessel until the display is stable for approximately 5 minutes Set the mode switch to the ZERO position With the INC DEC switch adjust the display for zero reading 2 Set the SP...

Page 21: ...sel base and the exhaust condenser with water lines 2 Carefully place the DC motor on the vessel assembly 3 Add glycerin to the thermowell and insert RTD temperature probe 4 Turn power to ON position...

Page 22: ...t up and use D O Mode proceed as follows 1 Set selector to Agitation 2 Set mode to set point 3 Adjust rpm with Inc Dec to the minimum rpm value you wish for D O control 4 Press D O control active and...

Page 23: ...gasket Use aseptic techniques 7 Repeat step 1 through 6 until desired number of samples are taken 3 12 SHUT DOWN PROCEDURES To shut down the system proceed as follows 1 Rotate flowmeter dial to zero 2...

Page 24: ...tions with all switches OFF and incoming power disconnected 1 Check the fuse s for clean contact 2 Check all controls and accessible items switches knobs fuse holders screws nuts and bolts to make sur...

Page 25: ...860 0130 DO Probe 1 25L Phoenix P0720 5440 DO Probe 2 5L Phoenix P0720 5450 DO Probe 5L Phoenix P0720 5460 DO Probe Cable P0720 2202 DO Probe 1 25L Ingold P0720 5565 DO Probe 2 5L Ingold P0720 5561 DO...

Page 26: ...ame Same DO Port P0280 5932 Same Same DO Port Ingold Washer M1016 0890 Same Same Teflon Washer Ingold DO P0100 9780 Same Same O Ring Ingold DO Probe P0280 9163 Same Same pH P0280 5912 Same Same 3 16 P...

Page 27: ...lowing through vessel EXHAUST CONDENSER 1 DESCRIPTION There are two quick connects on the condenser body the lower one has valve the upper one is without valve To connect the water lines connect the u...

Page 28: ...he antifoam pump is now on the bottom and the acid pump is on the top Switching pumps is achieved by switching electrical connections in the back of the unit Simply exchange the 4 plugs which go to on...

Page 29: ...terms have constants which are used in each term of the equation These constants have been picked based on New Brunswick Scientific s experience with this product and several cell lines The integral c...

Page 30: ...accurate control it became apparent that the ratio of flow rate or concentration to bioreactor volume was too high In this case control to 0 12 unit was obtained If the flow rate is increased still fu...

Page 31: ...ing and cooling times properly Most times this can be cured or improved by increasing the agitation and or airflow rates and thus improving the heat transfer Improper temperature control might also ha...

Page 32: ...Mode The fermentor responds to inquiry and modify set points commands Multi Drop Mode The fermentor responds to inquiry and modify set points commands which are prefixed with a unit number When using...

Page 33: ...for D and 0D is the code for carriage return The response of unit 1 to this inquiry is shown in Figure 1 0d A directive to change the setpoints of unit 1 is shown in Figure 1 0h EOL CHARACTERS The EOL...

Page 34: ...arly Figure 1 0c shows a typical response to a request for operating points ONE DIRECTIVE IS SUPPORTED BY THE FERMENTOR Modify Setpoints Figure 1 0g shows the required format for a Modify Set points d...

Page 35: ...FF 3 S1 4 is used for setting mode Mode S1 4 multidrop ON non multidrop OFF 4 S1 8 S1 7 S1 6 S1 5 are used for setting unit number Unit S1 8 S1 7 S1 6 S1 5 0 ON ON ON ON 1 ON ON ON OFF 2 ON ON OFF ON...

Page 36: ..._____________ multidrop parity adata parity bit __________________________________________________ multidrop no parity adata 1 __________________________________________________ non multidrop parity p...

Page 37: ...M1226 0050 30...

Page 38: ...RS232 Data Input to Fermentor 4 NC 5 NC 6 NC 7 GND Ground Reference for all Signal 8 NC 9 NC 10 NC 22 NC 11 NC 23 NC 12 IRXD RS422 Paired Data Input 24 IRXD to Fermentor 13 ITXD RS422 Paired Data Out...

Page 39: ...TABLE 5 HEXADECIMAL CODES FOR MULTIDROP UNIT NUMBERS Unit Number Hexadecimal Code 0 80 1 81 2 82 3 83 4 84 5 85 6 86 7 87 8 88 9 89 10 8A 11 8B 12 8C 13 8D 14 8E 15 8F M1226 0050 32...

Page 40: ...M1226 0050 33...

Page 41: ...1226 9206 Headplate 1 6c M1226 9208 Headplate 1 7a M1151 9905 Bumper Flat Gasket 2 7b M1152 9904 Bumper Flat Gasket 2 7c M1155 9902 Bumper Flat Gasket 2 8a M1151 9437 Clamping Ring 1 8b M1226 9426 Cla...

Page 42: ...ug 1 4 3 26 M1132 946T Ferrule 1 4 3 27 P0280 5302 O Ring 4 28 M1132 9433 Plug pH 1 29 M1132 9431 Cap 1 30 P0280 5912 O Ring 2 31 M1226 9418 Plug 1 2 1 32 H 727 Plug DO 1 33 P0280 5932 O Ring 1 34 M11...

Page 43: ...l Thermister 1 50 P0280 5362 O Ring 1 56 P0240 8270 Nut 1 57 P0240 7970 Ferrule 1 58 P0240 8260 Nut 1 59 P0240 7960 Ferrule 1 60 P0240 8240 Nut 3 61 P0240 7940 Ferrule 3 62 P0240 8230 Nut 1 63 P0240 7...

Page 44: ...ulation port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 fle...

Page 45: ...ulation port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 fle...

Page 46: ...port 2 M1132 946T Ferrule On port assy with tubing 6 M1226 9430 Ferrule 3 16 On 3 16 port assy with foam probe 2 P0240 7960 Ferrule Frt SS 3 8 tube On water in out jacket to console item 2 flexible h...

Page 47: ...nstallation 17 DO Probe Phoenix Installation 18 DO Probe Ingold Installation 19 Conversion of DO Port to Inoculation Port 20 Exhaust Condenser Installation 21 Restrictor in Condenser Water Line 22 Dis...

Page 48: ...FIGURE 1 VESSEL SCHEMATIC M1226 0050 41...

Page 49: ...FIGURE 2 SAMPLING SYSTEM M1226 0050 42...

Page 50: ...FIGURE 3 FLOWMETER CONVERSION M1226 0050 43...

Page 51: ...FIGURE 4 FRONT PANEL M1226 0050 44...

Page 52: ...FIGURE 5 REAR OF CABINET M1226 0050 45...

Page 53: ...FIGURE 6 SWITCH PANEL M1226 0050 46...

Page 54: ...FIGURE 7 EXTERNAL NUTRIENT PUMP TERMINAL M1226 0050 47...

Page 55: ...FIGURE 8 SUGGESTED IMPELLER LOCATION M1226 0050 48...

Page 56: ...FIGURE 9 HEADPLATE M1226 0050 49...

Page 57: ...FIGURE 10 SPARGER TUBE M1226 0050 50...

Page 58: ...FIGURE 11 HARVEST TUBE M1226 0050 51...

Page 59: ...FIGURE 12 THERMOWELL M1226 0050 52...

Page 60: ...FIGURE 13 FOAM PROBE M1226 0050 53...

Page 61: ...FIGURE 14 BAFFLE INSTALLATION M1226 0050 54...

Page 62: ...FIGURE 15 SIDE PORTS M1226 0050 55...

Page 63: ...FIGURE 16 pH PROBE INSTALLATION M1226 0050 56...

Page 64: ...FIGURE 17 D O PROBE PHOENIX INSTALLATION M1226 0050 57...

Page 65: ...FIGURE 18 D O PROBE INGOLD INSTALLATION M1226 0050 58...

Page 66: ...FIGURE 19 CONVERSION OF D O PORT TO INOCULATION PORT M1226 0050 59...

Page 67: ...FIGURE 20 EXHAUST CONDENSER INSTALLATION M1226 0050 60...

Page 68: ...FIGURE 21 RESTRICTOR IN CONDENSER WATER IN LINE M1226 0050 61...

Page 69: ...FIGURE 22 DISASSEMBLY OF CONDENSER M1226 0050 62...

Page 70: ...FIGURE 23 FACTOR AFFECTING pH CONTROL M1226 0050 63...

Page 71: ...J803 J804 J805 ADDRESS 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 9 10 11 8 7 4 5 1 2 3 RTDI RECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5...

Page 72: ...C B M1151 7008 J802 J801 J806 J803 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEP J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M11...

Page 73: ...AGIT ON MODE S001 J1 NC J2 1 2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE...

Page 74: ...OFF SI 8 1 3 2 0 ON ON ON ON 4800 9600 SI 6 SI 7 ON ON OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF SHIELD J1 3 PUMP M 100 VAC ON PRIME S009 D C 2 TB002 B A C VALVE TB002...

Page 75: ...7002 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH S...

Page 76: ...R M1151 4011 9 10 11 8 7 4 5 1 2 3 RTDI RECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 70...

Page 77: ...51 7008 J802 J801 J806 J803 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEPT J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009...

Page 78: ...S001 J1 NC J2 1 2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE RS232C MODE...

Page 79: ...800 9600 SI 6 SI 7 ON ON OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF CIRCULATION PUMP M SHIELD J1 3 WHT W BLK BLK C TB002 115 VAC PRIME S009 WHT VALVE TB002 BRN ON A B D...

Page 80: ...SE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3...

Page 81: ...ECEPTOR J1 AUTO S005 0N ANTIFOAM 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009 D O pH TRANSMITTER OFF OFF OFF OFF OFF...

Page 82: ...03 J804 J805 3 2 4 C 4 A B 1 TB001 ANTIFOAM DRIVER M1151 4011 RTD RECEPT J1 AUTO S005 0N ANTIFOAM PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTOR M1169 7009 D O pH TRANSMITTE TB001...

Page 83: ...2 3 4 5 M1153 BOARD DISPLAY 7002 NC NC 4 GND 4 GND 2 J4 5 3 J3 1 5 3 DECREASE INCREASE DO CONTROL 2 TACH 6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR AGITATION TEMPERATURE RS232C MODE CONTROL 2 1 SET...

Page 84: ...OFF OFF ON ON ON ON ON OFF SI 1 ON ON SWITCH BANK 1 1200 2400 SI 2 ON OFF CIRCULATION PUMP M SHIELD J1 3 WHT W BLK BLK C TB002 220 VAC PRIME S009 WHT VALVE TB002 BRN ON A B D C 2 4 W BLK BLK TB001 P0...

Page 85: ...6 7 250v 500mf M 5 J003 3 4 1 S002 SELECTOR 13 7 9 10 11 8 4 5 2 3 RTDI RECEPTOR J1 9 PH H 7 8 PH L 8 6 GND 5 7 5V 6 5 D0 L 8 4 D0 H 7 PH H E6 E7 PH L E5 PH SHIELD E1 E3 3 4 E2 E4 2 1 DO PROBE RECEPTO...

Page 86: ...M1226 0050 79...

Page 87: ...FIGURE 25 BEARING HOUSING ASSEMBLY M1226 0050 80...

Page 88: ...FIGURE 26 BEARING HOUSING IN STERILIZATION M1226 0050 81...

Page 89: ...M1226 0050 82...

Page 90: ...M1226 0050 83...

Page 91: ...M1226 0050 84...

Page 92: ...M1226 0050 85...

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NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

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