NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

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Check the level of the reference electrolyte. It should be about 1cm below the filling
orifice, which is closed with a rubber T stopper. To add reference electrolyte, take the
filling pipette (P0740-4820) and fill it with Viscolyte B (P0860-0130) Electrolyte.
NOTE: The two chambers are filled with same Reference Electrolyte.
The total volume of Reference Electrolyte held be the electrode
is approximately 30mL. During normal operation the two rubber
stoppers are to be removed.
3.6
pH ELECTRODE PROBE CALIBRATION
pH probe is to be calibrated prior to autoclaving vessel.
Connect electrode to probe cable.
Turn the agitation switch OFF.
Turn ON power switch.
NOTE: The pH measuring system is calibrated using two buffer
solutions of a known pH.
1. Set the selector switch to pH.
Set the mode switch to "ZERO".
Immerse the pH probe into an external pH 7.00 buffer solution.
Set the display to read the pH value of the buffer with INC/DEC switch.
2.
Immerse the pH electrode in a second external buffer solution which is several
pH units above or below the pH selected in the previous step.
Set the mode switch to "SPAN".
Set the display to read the value of the second buffer solution with the INC/
DEC switch.
NOTE: After autoclaving it is recommended that the calibration is
checked by taking a sample from the vessel and measuring
the pH with an external pH meter.
M1226-0050 12