NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

Page: 8 / 92

CHAPTER 1
INTRODUCTION
1.1 SCOPE OF MANUAL
This manual contains a description of the vessel, installation, operating and
maintenance instruction for the BioFlo III System 1.25, 2.5 and 5L Models
manufactured by the New Brunswick Scientific Co., Inc., 44 Talmadge Road,
P.O. Box 4005, Edison, New Jersey 08818-4005, USA.
1.2 DESCRIPTION OF EQUIPMENT
BioFlo III is a versatile bioreactor that provides a fully equipped state of the art
fermentation system in one compact package. BioFlo III can be employed for batch
or continuous culture with microprocessor control of pH, DO, agitation, temperature,
nutrient feed, and electronic foam control.
1.3 DESCRIPTION OF VESSEL
The vessel parts consist of a stainless steel head plate, a flanged glass tube (thick
walled) vessel body which is detachable from the bottom dished head. The dished
head is jacketed for circulation of temperature controlled water. Four sterilizable
polypropylene compression ports are provided in the glass wall for the addition of
antifoam and nutrients, as well as for vessel overflow in continuous culture studies.
Ports are provided in the headplate for: Inoculation, base and acid addition, a
thermowell for Resistance Temperature Detector, a Sparger, a Harvest Tube, a
Sampler, an Exhaust Condenser, Dissolved Oxygen and pH Electrodes. The drive
bearing housing is also located on the headplate.
1.4 AGITATION SYSTEM
A removable agitation motor located on top of the bearing housing is connected to the
agitation shaft with a multi-jaw coupling. It can be easily disconnected while
autoclaving the vessel and replaced after sterilization. The motor will provide
agitation
speed range of 50 to 1000
±
(2 RPM + 0.5% setting).
1.5 TEMPERATURE CONTROL
The culture temperature may be selected in the range from 4
°
C to 80
°
C (
±
0.1
°
C) and
is controlled by a microprocessor based PI (Proportional and Integral) controller. The
media temperature is sensed by an RTD (Resistance Temperature Detector)
submerged
in the thermowell.
M1226-0050 1