NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

Page: 16 / 92

before inserting into the port in the headplate.****
12. Insert exhaust condenser into the exhaust condenser port. Connect the
exhaust filter on the top of the condenser with flexible tubing (Fig. 20).
13. Slide 2" long, 1/4" inside diameter silicone tubing on the top of the sparger
tube, then connect air filter to it (Fig. 10).
NOTES:
* Add a few drops of glycerin into the thermowell tube before inserting
RTD.
** Do not overtighten the clamping ring to the headplate. Same to the
clamping screws for the glass tube reactor and the vessel base
(vessel heater).
*** When the inoculation port is used for installation of Phoenix DO
probe, the DO port is converted to the inoculation port (Fig. 19).
**** Membrane of the probe must be free of glycerin.
3.3 PREPARATION BEFORE STERILIZATION
Before sterilization, perform the following procedure:
1. Fill vessel with water to its working volume.
2. Place the vessel assembly on the three pins located in the base of the cabinet.
3. Position the motor assembly on the top of the bearing housing. Connect the
motor cable to the receptacle on the face of the cabinet.
4. Connect cables from all probes (pH, DO and antifoam) to their respective
sockets on the front face of the cabinet.
Ground lead from the antifoam socket is to be connected to the ground pin
on the headplate.
5. Insert RTD in the thermowell on the headplate and connect it to the socket
on the cabinet.
6. Connect the inlet filter on the top of the sparger tube to the hose barb in the
face of the cabinet with flexible tubing.
7. Connect water line to rear of the cabinet. Adjust water pressure to 20 PSIG
max.
M1226-0050 9