NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

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CHAPTER 3
PREPARATION AND OPERATION
3.1 CLEANING OF VESSEL
1. Fill the vessel with a mild detergent and water solution. Let stand for one hour
then scour thoroughly. Use a brush on both inside and outside surfaces.
2. Drain the vessel and rinse several times with tap water. Repeat rinsing with
distilled water and let dry.
3.2 VESSEL ASSEMBLY
1. Slide impellers on the shaft of the bearing housing and clamp them down.
Lower impeller should be positioned about 1/4 inch above the bottom of the
baffle. Upper impeller one to one and half impeller diameters above lower
impeller (Fig. 8).
Working From the Underside of the Headplate (Fig. 9)
2. Insert the sparger tube in the sparger port (Fig. 10).
3. Insert the harvest tube in the harvest port (Fig. 11).
Working From the Top of the Headplate
4. Insert the thermowell tube into the temperature port* (Fig. 12).
5. Insert the sampler assembly into the sample port (Fig. 2).
6. Install foam probe in the headplate (Fig. 13).
7. Install the baffle assembly inside of the glass jar by pressing the two edged
baffle together (Fig. 14).
8. Install four polypropylene ports or plugs in the wall of the glass jar. Be sure to
use a blind plug when a port is not in use (Fig. 15).
9. Lubricate the headplate O-ring with silicone grease.
10. Place the headplate on the flange of the vessel and lock it to the clamping ring
with knurled screws.**
11. Install pH and DO probes (Fig. 16, 17, 18).*** Coat probe with glycerin
M1226-0050 8