NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

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3.7 DISSOLVED OXYGEN PROBE CALIBRATION
Probe is to be calibrated after autoclaving vessel.
1.
There are two methods of obtaining zero on DO.
METHOD 1 (Less Accurate)
Remove the DO probe cable from the DO probe. Set the selector switch to
"DO".
Set the mode switch to "ZERO". With the INC/DEC switch adjust the display
reading on the DO to read zero. Connect the DO probe cable to the probe.
METHOD 2
Connect the DO probe cable to the DO probe. Set the selector to DO.
Set the agitation speed to 500 RPM.
Sparge Nitrogen into the vessel until the display is stable for approximately
5 minutes.
Set the mode switch to the "ZERO" position.
With the INC/DEC switch adjust the display for zero reading.
2.
Set the "SPAN".
Set the selector to DO.
Set the mode switch to "SPAN". Vigorously sparge air into the vessel. Set the
agitation speed to 500 RPM.
After about 30 minutes observe the reading on the display. After the DO
reading has stabilized. With the INC/DEC switch adjust the DO display
reading
to
100.
3.8 SELECTION OF MEDIUM FLOW RATE
Medium flow rate depends on the bore size of the tubing and percentage of the time
that nutrient pump will be running (at constant speed).
Nutrient Pump (Seconds):
M1226-0050 13