NEW BRUNSWICK SCIENTIFIC BIOFLO III, Manual To Operations

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INSTALL A DOUBLE FILTER SYSTEM
Double exhaust and double inlet filters are recommended.
•
Attach a "Y" fitting to the top of the condenser with a piece of tubing. Attach
an exhaust filter on each branch. This allows you the flexibility to exchange
sterilized filters during a run should one filter become clogged, (pinch off
unused line with a clamp).
NOTE: Fifty generations of recombinant E. coli have been propagated
by BioFlo III in continuous culture. Prolonged operation as a
chemostat requires double filters.
OPERATION AS A CHEMOSTAT, SUGGESTIONS
Switch the relative positions of the antifoam and acid pumps on the console so that the
antifoam pump is now on the bottom and the acid pump is on the top.
•
"Switching" pumps is achieved by switching electrical connections in the back
of the unit.
•
Simply exchange the 4 plugs which go to one pump board with the 4 plugs
from the other pump board.
•
It is convenient to use the newly positioned antifoam pump as the harvest
pump, (gravity is now in your favor; a continuously filled harvest tube will
prevent "grow back").
•
Place L-shaped harvest tube through a side port, 3/4 of the way down to
the bottom of the vessel.
Use the antifoam probe as a level probe to actuate the harvest pump.
OTR CALCULATIONS
OTR can be estimated by titrating fixed amount of sodium sulfite, Na2SO3, with
air,
•
2SO3 + O2 CU+2 2SO4
Calibrate D.O. electrode.
•
Set zero on DO
Fully oxygenate fermentor with agitation and airflow.
•
Set span to 100%
M1226-0050 21