Molecular Devices QPix 420, User Manual

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QPix 420 Colony Picking System User Guide
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To undo all selections, click the Remove User Selections icon to display the Remove User
Selections dialog. Click Yes.
If you run a routine for white light and fluorescent imaging, two tabs are available.
The White Light tab displays the image taken with white light.
The other tab is labeled with the fluorescent filter pair and displays the image taken with
fluorescent light.
Switch between the two images to define the pickable colonies. This option is available only for
instruments with a fluorescence imaging module.
To select the colonies for picking:
1. From the list in the upper-right area, select the barcode or identifier of the receptacle to
view.
2. Refine the colony Selection criteria.
Compactness sets the level of irregularity for picking colonies. The value is a ratio of
the perimeter divided by the area of the colony, so that irregular shaped colonies are
closer to 0 and colonies that are more of a perfect circle are closer to 1. A colony equal
to or less than 0.65 is not picked by default.
Axis Ratio measures how oval the colony is. The value is a ratio of the longest diameter
divided by the shortest diameter, so oval shaped colonies is closer to 0 and round
colonies is closer to 1. A colony equal to or less than 0.65 is not picked by default.
Min Diameter (mm) sets the minimum diameter of colonies for picking. A colony equal
to or less than the value in this field is not picked.
Note: The Min Diameter (mm) cannot be lower than the Diameter value set in the
Debris Discard section of the test image.
Max Diameter (mm) sets the maximum diameter of colonies for picking. A colony equal
to or greater than the value in this field is not picked.
Min Proximity (mm) sets the distance between colonies to be picked, so that when
picking one colony, a different adjacent colony is not picked. The system default value
is 0.45 mm.