Molecular Devices QPix 420, User Manual

Page: 170 / 225

QPix 420 Colony Picking System User Guide
170 5031496 G
10. To reduce the pin-firing speed, select the Slow Pin Fire check box.
Reducing the pin-firing speed can prevent cross-contamination due to occasional splashing
that can occur with normal pin-firing speed. However, this significantly increases the
amount of time required for the process.
11. In the Microplates to Process Before Depositing field, enter the number of source plates
from which to pick colonies before depositing the colonies into the destination plates.
12. Click Next to set the destination source options.
Selecting the Destination Plate Options
To select the destination plate options:
1. In the Destination window, from the Select Destination Microplate list, select the plate type
to receive the colonies picked from the source plates. If the plate type that you need is not
listed, contact Molecular Devices to add a new plate type to the list. See
Obtaining Support
on page 209 .
2. Select either to dip the pins a number of times or to stir the wells of the destination plate
during inoculation.
To dip the pins a number of times, click Multi Dip and in the Number of Dips field, enter
the number of times to dip the pins.
To stir the wells, click Stir Destination.
3. Select the Deposit Order to deposit the picked colonies By Columns or By Rows.
4. Under Destination Microplate Template, click Edit to define the wells to dip or skip. You can
skip wells that you want to use as blank or control wells. The defined template is used for all
the destination plates during the rearraying routine.
To skip a well, click the well. Wells to skip display in light blue.
To skip multiple contiguous wells, right-click and drag across the wells.
To dip a well that you select to skip, click the well again. Wells to dip display in light red.
After defining the wells to dip or skip, click Exit.
5. Click Next to select the head options.