Molecular Devices QPix 420, User Manual

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QPix 420 Colony Picking System User Guide
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4. Select the Use Auto Thresholding check box to have the software automatically detect the
colonies in the image.
To manually detect the colonies, clear this check box and drag the slider until all the
desired colonies are detected and the background is not.
5. Select the Auto Threshold Each Frame check box to process each frame with its own
uniquely calculated value.
To process the frames using a value calculated of the entire image, clear this check box.
6. With the Auto Threshold Each Frame check box selected, enter threshold values in the Low
Limit and High Limit fields.
To determine the Low Limit value, clear the Use Auto Thresholding check box and drag
the slider to the left until some background is clearly detected. Select the Use Auto
Thresholding check box and the Auto Threshold Each Frame check box again and
then enter the value from the Threshold field into the Low Limit field.
To determine the High Limit value, clear the Use Auto Thresholding check box and
then drag the slider to the right until some colonies start to become undetected. Select
the Use Auto Thresholding check box and the Auto Threshold Each Frame check box
again and then enter the value from the Threshold field into the High Limit field.
7. Select the Invert Image check box to make dark areas bright and bright areas dark.
8. Select the Subtract Background check box to have the background become nearly black.
9. Select the Display tab.
10. Select the method for viewing the detected colonies in the image.
Click Image Only to display the detected colonies in white with yellow rings and a gray
background.
Click Image and Overlay to display the detected colonies in green with yellow rings
and a gray background.
Click Overlay only to display the detected colonies in white with yellow rings and a
black background.
To remove the yellow ring from the detected colonies, clear the Outline Detected Features
check box.
11. Select the Debris tab.