Molecular Devices QPix 420, User Manual

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QPix 420 Colony Picking System User Guide
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11. Select the Debris tab.
12. Adjust the Diameter and Axis Ratio to exclude objects that are smaller than the desired
colonies. Each detected feature displays within a yellow ring while excluded features do not
have a yellow ring.
In the Diameter (mm) field enter a value in millimeters for the minimum diameter of the
required colonies.
In the Axis Ratio field, define the minimum roundness ratio of the required colonies.
13. Select the Depth tab.
14. Adjust the Agar Depth.
In the Agar Depth (mm) field, enter a value in millimeters for the depth that the picking
head descends into the source agar.
The Agar Height (mm) field is read only and displays the height, or thickness, of the
detected agar within the source tray.
The Max. Agar Height (mm) field is read only and displays the maximum agar height for
a long pin head to descend into the source agar
Note: Further refinement for colony detection can be done on a higher-resolution
image in the Feature Selection window.
15. Click Next to go to the next step.
If you run a routine for white light only, the system captures and processes a higher-
resolution image and then displays the Feature Selection window.
If you run a routine for white light and fluorescent imaging, the Fluorescent Test Image
window displays.
Adjusting the Fluorescent Test Image
If you run a routine for white light and fluorescent imaging, the Fluorescent Test Image window
displays after you make adjustments for the white light test image. This option is available only
for instruments with a fluorescence imaging module.
To view the pixel intensity values in the image map to manually set a detection threshold or
detect areas of saturation within the image:
1. Right-click over the image map and select Intensity.
2. Move the cursor over the area of interest in the image map to display the pixel intensity
value.