MJ Research PTC-0200, Operation Manual

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DNA Engine & DNA Engine Tetrad Operations Manual
Editing a gradient step
To edit a gradient step, select Edit from the Main Menu. The program
will be displayed as follows:
List: CUSTOM1
1= 92.0
°
for 0:20
2= 50.0
°
to 70.0
°
for 0:30
Use the « Select » keys to scroll to the step you want to edit.
Reviewing a gradient program
After the protocol has been run, the Program Complete screen will be dis-
played. Press the «
Proceed
» key, and the next screen will display a re-
view of the gradient well temperatures:
Gradient Review:
Column 1- 50.0
°
left
Column 2- 50.5
°
Column 3- 51.5
°
Column 4- 53.2
°
Column 5- 55.5
°
Column 6- 58.4
°
Column 7- 61.8
°
Column 8- 64.6
°
Column 9- 66.8
°
Column 10- 68.4
°
Column 11- 69.6
°
Column 12- 70.0
°
right
Scroll up and down the screen using the «
Select
» keys. When scrolling
the “Gradient Review” line will be held constant while the lower 3 lines
can be manipulated up or down. The temperatures displayed will be for
each of the twelve wells along the long-axis of the sample block. These
temperatures will be predicted from an algorithm as a steady-state tem-
perature of the block and the sample. You may then return to the main
menu by pressing the «
Proceed
» key.
Using the gradient calculator
The gradient calculator predicts, for a given gradient, the temperatures
for each of the twelve wells along the long-axis of the sample block. These
temperatures will be predicted from an algorithm as a steady-state tem-
perature of the block and the sample. To use the gradient calculator,
select the
List command from the main menu and then press the « Pro-
ceed » key. You will see a List screen: