5-5
5-5
5-5
5-5
5-5
Running Protocols
Setting Up a Probe-Control Protocol
A single screen asking about use of the heated lid will be displayed. Se-
lect
Yes
or
No
, then press «
Proceed
». If the probe is installed, the probe
verification screen will be displayed:
Ensure that the probe is correctly installed (see “Loading and Connecting the
Probe,” chapter 4), then press «
Proceed
». The protocol will begin running.
If the probe is not installed, a warning message will be displayed:
This screen says that since the probe is missing, the DNA Engine will
run the protocol using calculated control if «
Proceed
» is pressed now. To
avoid this, press «
Cancel
», install the probe, and set the protocol up again.
When the probe verification screen is displayed, press «
Proceed
» to be-
gin the protocol run.
Reading the Runtime Screen
During a protocol run a runtime screen will be displayed:
The screen lists the protocol name (2-STEP in the example above), proto-
col step that is running (1 ), cycle number (1), method of temperature
control (Calc), and the block temperature for block-control protocols, the
calculated sample temperature for calculated-control protocols (68.0), and
the probe temperature for probe-control protocols. When the step’s tar-
get temperature is reached, a timer begins running in the lower right-
Run: PROBE1
PROBE control:
Verify probe ready
RUN: PROBE1
CALC control:
Probe not present
Run: 2-STEP
1= 92.0˚ for 0:05
Cycle: 1
Calc: 68.0˚
Summary of Contents for PTC-0200
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