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On the left of the window are two controls that are
misleadingly labeled. They are, effectively, coarse (left)
and fine (right) control of laser intensity at the
specimen. The number at the base of the left slider is the
excitation wavelength, in nm.
Best practice is initially to set the coarse slider to zero
and the fine slider to a low value. Using the Live mode,
increment the coarse slider slowly until an image is
obtained. Adjust the fine slider to obtain the optimum
image.
