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Pinhole
The pinhole is not in the emission path for the non-descanned detectors, so it can be ignored. Operation
of the confocal with multi-photon excitation and descanned detection is ineffective and should be
avoided.
Enabling the MP Laser
Unlike the semiconductor lasers, the MP laser must be
turned on in the
Configuration
pane.
Setting up the MP Laser
In the
Acquire
pane, first click MP on. Then click on the ‘+’ button to
open the MP laser window.
The MP Laser Window
Select the laser wavelength. The laser will take 2-3
minutes to go from ‘CW”, with little or no power, to
‘pulsing’, with full power (2-3 W). T0 open the shutter,
press and hold the shutter button.
