12
LAS AF Application
The application screen will start up in the Acquire pane and will look like this:
Buttons and Sliders
Most functions are controlled by sliders. Click on the
slider button to change the slider value. Drag the button
or, for more precise adjustment, use the mouse wheel.
Some functions also allow keyboard data entry.
Confocal Controller
The confocal controller has six knobs that control
acquisition functions and two buttons that toggle the
current image/ detector combination. The knobs and
buttons can be operated while acquiring in
Live
mode.
Modifications to them will be reflected in the Acquire
pane. From left to right they are:
Left button – move the current detector/image one step to the left.
Right button - move the current detector/image one step to the right.
Smart Gain – adjust the gain of the current detector.
Smart Offset - adjust the offset of the current detector (if it is a PMT).
Scan Field Rotation - self-explanatory.
Pinhole Diameter (in Airy units)–use sparingly.
Zoom –a large range is available (0.7 to 40). An audible warning will sound when the usable
zoom has been exceeded.
Z position (in µm) – particularly useful when setting up stacks.
