15
Acquisition Rate
The acquisition rate window selects the acquisition scan
parameters, including the number of pixels in the image
and the scan speed, via pull-down menus. We
recommend 400 Hz as an initial rate.
The image scan may be zoomed down to 0.7 and up to
20.
Averaging may be by line or by frame, using pull-down
menus – we recommend line averaging and no more
than 4 averages.
The scan orientation may be controlled using the
rotation slider. The image area may also be translated in
the directions indicated by the rosette arrows. Large
lateral translations at low zoom will move the scan range
off axis and are therefore not recommended. Move the
stage instead.
Pinhole
There is only one pinhole. Its diameter is determined by
the longest emission band and defaults to 1 Airy disk. It
may be modified by clicking and dragging on the pinhole
button.
Lasers
The visible light lasers must be enabled by clicking on
the ON/OFF button. The lasers may be selected using
the laser menu in the Configuration Plane, or by clicking
on the ‘+’ sign. Click and drag on the button to adjust
power level.
