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Alternate Acquisition Modes - Tile Scan
Select Tile Scan in the Acquisition Mode menu. The Tile Scan
window will appear below in the Acquisition window.
Note: Tile Scan should not be combined with image rotation or
erroneous results will be obtained.
Tile Scan Window
The Tile Scan window features an X-Y map showing the current
position of the stage. The scale is in mm, and the X-axis is
vertical, as for the image display. The map scale can be
expanded or contracted using the button and slider below the
map.
Above the map are four buttons. The left-most button
selects the current position for storage. Multiple positions can
be stored. The current position number is reflected below the
map. The second button deletes the current stored position, as
reflected by the position number below the map. The third
button deletes all currently-stored positions. Clicking on the
right-most button opens a stage configuration menu
The simplest method is to select Auto Stitching, with
Smooth off.
Stage Configuration Menu
Note that in the default condition, the X- and Y- axes are
inverted in sign (corresponding to the inverted
microscope image), and that the X and Y axes are
exchanged.
When Auto Stitching is selected, the percent of
overlap is selected in this menu. We find that 10%
overlap works satisfactorily without requiring excessive
sampling or computation time.
