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Dispersion Compensation
Dispersion compensation of the near-IR laser is set in the GDD
pull-down panel. Select the appropriate objective form the table.
The default (zero GDD) is adequate for multi-photon operation.
The fine tuning slider can be adjusted for special circumstances
(e.g., operation in deep tissue).
Setting the NDD Detectors
Starting up in MP_LASER_ON mode will enable the NDD
Super HyD detectors. These are controlled using a single gain
slider. The NDD detectors are
very sensitive.
Use low gain and
low laser power initially. Operate only in a darkened room. A
darkroom cloth is available to mask stray light.
Managing MP Laser Power
The EOM window, which controls MP laser power, has
four slider controls.
On the right, the EOM slider must be set to 1 to permit
near-IR excitation to reach the specimen. The offset
parameter is set by the application as a function of
wavelength and should not be modified.
