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10. Contrasting Methods for Leica DM6000 M
10.2 Transmitted Light
10.2.1 Bright Field (TL)
• Switch to the transmitted light axis by pushing
the
TL/IL
button.
• Select the
BF
(bright field) contrast method.
Do so by pressing the
BF TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display of the LeicaScreen, menu page
‘Status’ indicates
BF TL
.
• Insert a transmitted light specimen.
• Select an appropriate objective.
• Focus on the image with the turning knob on
the SmartMove or with the focus wheel and
adjust the illumination with the
INT
-keys.
10.2.2 Polarization (TL)
• Switch to the transmitted light axis (TL) by
pushing the
TL/IL
button.
• Select the
POL
(polarization) contrast method.
Do so by pressing the
POL
variable key.
Alternatively: Press the
CHANGE TL
variable key.
(For key occupation please see “Identification
Sheet”.)
The display of the LeicaScreen, menu page
‘Status’ indicates
POL
.
Manual procedure:
• Turn the polarizer on the underside of the
condenser in the light path (Fig. 75). Make
sure that the red index point on the front of
the polarizer is aligned with 0.
• Insert the analyzer into the left side of the
stand (76.1).
• Bring the polarizer and analyzer into cross po-
sition until they reach maximum darkness.
• Insert a specimen and select a low-
magnification objective.
Motorized procedure:
• After selecting the
POL
contrast method, the
condenser automatically switches to the posi-
tion of the polarizer. The analyzer cube is also
automatically brought into the light path.
Combined procedure:
• For the Leica DM6000 M microscope, it is
possible to combine mechanical and
motorized components.
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