SMART Spectro 2 Operator’s Manual 04.16
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Sample Color
Wavelength Range
Yellow
350-450
Yellow-Orange
450-490
Orange
490-510
Pink
510-570
Red
570-600
Green and Blue
600-750
CALIBRATION CURVES
The SMART Spectro 2 contains precalibrated tests for the LaMotte reagent systems. The
fi rst step in using a non-LaMotte reagent system with the SMART Spectro 2 is to create a
calibration curve for the reagent system. To create a calibration curve, prepare standard
solutions of the test factor and use the reagent system to test the standard solutions with
the SMART Spectro 2.
Plot the results (in ABS or %Transmittance) versus concentration to create a calibration
curve. The calibration curve may then be used to identify the concentration of an unknown
sample by testing the unknown, reading Absorbance or %T, and fi nding the corresponding
concentration from the curve. The linear range of the reagent system can be determined
and this information can be used to input a User Test into the SMART Spectro 2 (see EDIT
USER TESTS, page 58).
PROCEDURE
1. Prepare 5 or 6 standard solutions of the factor being tested. The concentration of
these standards should be evenly distributed throughout the range of the reagent
system, and should include a 0 ppm standard (distilled water). For instance, the
solutions could measure 0, 10%, 30%, 50%, 70%, and 90% of the system’s maximum
range.
2. Turn on the SMART Spectro 2. Select the appropriate %T/ABS wavelength from the
%T/ABS mode. Be sure to select the appropriate wavelength for the color produced by
the reagent system.
3. Use the unreacted 0 ppm standard to standardize the spectrophotometer by using it
to scan blank.
4. Following the individual reagent system instructions, react each standard solution
including 0 ppm. Record the reading and the standard solution concentration on a
chart. Readings can be recorded as percent transmittance (%T) or absorbance (A).
5. Plot results on graph paper or computer using any available plotting program. If
results are as %T versus concentration, semilog graph paper must be used. Plot
the standard solution concentrations on the horizontal, linear axis, and the %T on
the vertical, logarithmic axis. If results are as absorbance versus standard solution
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