Test Procedures
PROCEDURE–FREE CHLORINE
Use universal sample holder
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
.
3. Scroll to and select
ALL TESTS
(or another sequence containing
17 Cl Liquid DPD
) from
TESTING MENU
.
4. Scroll to and select
17 Cl Liquid DPD
from menu.
5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample.
6. Insert the tube into chamber, close lid and select
SCAN BLANK
.
7.
Remove the tube from spectrophotometer.
8. Add 5 drops of DPD 1A Free Chlorine Reagent (P-6740).
9. Add 5 drops of *DPD 1B Free Chlorine Reagent (P-6741). Cap and mix.
10. Insert tube into chamber, close lid and select
SCAN SAMPLE
. Record result as ppm
free chlorine.
PROCEDURE–TOTAL CHLORINE
11. Add 5 drops of DPD 3 Total Chlorine Reagent (P-6743). Cap and mix.
NOTE: For wastewater samples, Standard Methods for the Examination of Water and
Wastewater recommends waiting 2 minutes for full color development.
12. Insert tube into chamber, close lid and select
SCAN SAMPLE
. Record result as ppm
total chlorine.
13. Subtract the Free Chlorine reading from the Total Chlorine reading to determine ppm
combined chlorine.
14. Press and hold
OFF
button to turn the spectrophotometer off or press
EXIT
button to
exit to a previous menu or make another menu selection.
NOTE: For best possible results, a reagent blank should be determined to account for any
contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
SMART Spectro 2 Test Procedures 05.02.16
CHLORINE,
LIQUID
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