Test Procedures
PROCEDURE
Use universal sample holder.
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
.
3. Scroll to and select
ALL TESTS
(or another sequence containing
5 Ammonia-N H
) from
TESTING MENU
.
4. Scroll to and select
5 Ammonia-N H
from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.
6. Insert tube into chamber, close lid and select
SCAN BLANK
. (See Note)
7.
Remove tube from Spectro. Add 8 drops of Ammonia Nitrogen Reagent #1 (V-4797).
Cap and mix. Wait 1 minute.
8. Use the 1.0 mL pipet (0354) to add 1.0 mL of *Ammonia Nitrogen Reagent #2 (V-
4798). Cap and mix. Allow 5 minutes for maximum color development.
9. At end of 5 minute waiting period, mix, insert tube into chamber, close lid and select
SCAN SAMPLE
. Record result.
10. Press and hold
OFF
button to turn the spectrophotometer off or press the
EXIT
button
exit to a previous menu or make another menu selection.
CALCULATIONS:
To express results as Unionized Ammonia (NH
3
):
ppm Unionized Ammonia (NH
3
) =
ppm Ammonia-Nitrogen (NH
3
–N) x 1.2
To express results as Ionized Ammonia (NH
4
):
ppm Ionized Ammonia (NH
4+
) =
ppm Ammonia-Nitrogen (NH
3
–N) x 1.3
NOTES: For the best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult
the Appendix.
AMMONIA-NITROGEN,
High Range
SMART Spectro 2 Test Procedures 05.02.16
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