Test Procedures
PROCEDURE
Use universal sample holder
1. Preheat COD reactor to 150 ±2°C. Follow safety precautions.
2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet
(0354) to add 5.0 mL of sample.
3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of
*Digestion Reagent Powder (4036). Tap funnel to dispense powder completely. Cap
tube tightly and shake until powder dissolves completely.
4. Place the tube in the COD reactor for 30 minutes.
5. At the end of the heating period, turn the reactor off . Carefully remove the tube from
the reactor and allow it to cool to room temperature.
6. At the end of the cooling period, press
ON
to turn spectrophotometer on.
7.
Scroll to and select
PROGRAMMED TESTS
from menu.
8. Scroll to and select
ALL TESTS
(or another sequence containing
82 Phosphorus T LR
from
PROGRAMMED TESTS
menu.
9. Scroll to and select
82 Phosphorus T LR
from the menu.
10. Carefully remove the caps from the digested tube. Use another 1 mL pipet (0354) to
add 1.0 mL of *Total Phosphorus LR Hydroxide Reagent (4038) to the tube. Cap and
invert to mix.
11. Wipe the vial with a damp towel to remove fi ngerprints and smudges. Wipe with a dry
towel.
12. Insert the tube into the chamber. Select
SCAN BLANK
. Remove the tube from the
spectrophotometer.
13. Use another 1 mL pipet (0354) to add *1.0 mL of Phosphate Acid Reagent (V-6282).
Cap and invert tube to mix.
14. Use the 0.1g spoon (0699) and a funnel (0459) to add one level spoon of Phosphate
Reducing Reagent (V-6283). Tap funnel to dispense powder completely. Cap tube and
shake until powder dissolves.
15. Wait 5 minutes.
16. Wipe the tubes with a damp towel to remove fi ngerprints and smudges. Wipe with a
dry towel.
17. Insert the tube into the chamber. Select
SCAN SAMPLE
. Record the result as Total
Phosphorus in mg/L PO
4
.
18. Press and hold
OFF
button to turn the spectrophotometer off or press
EXIT
button to
exit to a previous menu or make another menu selection.
NOTE: For greater accuracy, use laboratory grade pipets.
SMART Spectro 2 Test Procedures 05.02.16
PHOSPHORUS
, TOTAL, LOW RANGE
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