Kendro SORVALL Biofuge primo, Operating Instructions Manual

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Operation
30
Loading the rotor
Maximum loading
Overloading may cause the rotor to
explode! Exploding parts may se-
verely damage the centrifuge!
The Biofuge primo can reach high rotational speeds
implying enormous centrifugal force. The rotors are
designed in a way warranting sufficient residual
strength even at the highest permissible speed.
However, this safety system presupposes that the
maximum permissible load of the rotor is not ex-
ceeded.
If you wish to centrifuge samples that together with the
adapters exceed the maximum permissible load, you
must either reduce the sample volume or calculate the
permissible speed n
perm
according to the following for-
mula:
n
perm
n
=
∗
max
maximum permissible load
actual load
Filling the centrifuge tubes
Please note that plastic sample vessels only
have a limited service life - particularly when
used at maximum rpm or temperature - and
must be replaced as necessary!
Check carefully whether your sam-
ple vessels are permissible for the
respective
g value and reduce the
speed if necessary.
The smaller the unbalance of the centrifuge, the better
the separation since separated zones are no longer
perturbed by vibration. It is therefore important to bal-
ance the centrifuge tubes as well as possible.
To minimize unbalance you should fill the tubes as
evenly as possible. You can achieve this by eye. How-
ever, you must nonetheless ensure that opposite tubes
are filled to the same level.